A functional survey of the enhancer activity of conserved non-coding sequences from vertebrate Iroquois cluster gene deserts

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dc.contributor.authorDe La Calle-Mustienes, E.
dc.contributor.authorFeijoo, C.
dc.contributor.authorManzanares, M.
dc.contributor.authorTena, J.
dc.contributor.authorRodriguez-Seguel, E.
dc.contributor.authorLetizia, A.
dc.contributor.authorAllende, M.
dc.contributor.authorGomez-Skarmeta, J.
dc.date.accessioned2021-06-16T19:40:29Z
dc.date.available2021-06-16T19:40:29Z
dc.date.issued2005-08
dc.descriptionIndexación: Scopus.es
dc.description.abstractRecent studies of the genome architecture of vertebrates have uncovered two unforeseen aspects of its organization. First, large regions of the genome, called gene deserts, are devoid of protein-coding sequences and have no obvious biological role. Second, comparative genomics has highlighted the existence of an array of highly conserved non-coding regions (HCNRs) in all vertebrates. Most surprisingly, these structural features are strongly associated with genes that have essential functions during development. Among these, the vertebrate Iroquois (Irx) genes stand out on both fronts. Mammalian Irx genes are organized in two clusters (IrxA and IrxB) that span >1 Mb each with no other genes interspersed. Additionally, a large number of HCNRs exist within Irx clusters. We have systematically examined the enhancer activity of HCNRs from the IrxB cluster using transgenic Xenopus and zebrafish embryos. Most of these HCNRs are active in subdomains of endogenous Irx expression, and some are candidates to contain shared enhancers of neighboring genes, which could explain the evolutionary conservation of Irx clusters. Furthermore, HCNRs present in tetrapod IrxB but not in fish may be responsible for novel Irx expression domains that appeared after their divergence. Finally, we have performed a more detailed analysis on two IrxB ultraconserved non-coding regions (UCRs) duplicated in IrxA clusters in similar relative positions. These four regions share a core region highly conserved among all of them and drive expression in similar domains. However, inter-species conserved sequences surrounding the core, specific for each of these UCRs, are able to modulate their expression. ©2005 by Cold Spring Harbor Laboratory Press.es
dc.description.urihttps://genome.cshlp.org/content/15/8/1061
dc.identifier.citationGenome Research, Volume 15, Issue 8, Pages 1061 - 1072, August 2005es
dc.identifier.doiDOI: 10.1101/gr.4004805
dc.identifier.issn1088-9051
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/19126
dc.language.isoenes
dc.publisherCold Spring Harbor Laboratory Presses
dc.subjectEnhancer Regiones
dc.subjectDevelopmental Geneses
dc.subjectRegulatory Sequenceses
dc.subjectGenetically Modifiedes
dc.subjectBase Sequencees
dc.subjectConserved Sequencees
dc.subjectIntergenices
dc.subjectDNAes
dc.titleA functional survey of the enhancer activity of conserved non-coding sequences from vertebrate Iroquois cluster gene desertses
dc.typeArtículoes
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