Frizzled-1 receptor regulates adult hippocampal neurogenesis

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dc.contributor.authorMardones, M.D.
dc.contributor.authorAndaur, G.A.
dc.contributor.authorVaras-Godoy, M.
dc.contributor.authorHenriquez, J.F.
dc.contributor.authorSalech, F.
dc.contributor.authorBehrens, M.I.
dc.contributor.authorCouve, A.
dc.contributor.authorInestrosa, N.C.
dc.contributor.authorVarela-Nallar, L.
dc.date.accessioned2017-08-18T18:51:55Z
dc.date.available2017-08-18T18:51:55Z
dc.date.issued2016-03
dc.descriptionIndexación: Web of Science; Scopus.es_CL
dc.description.abstractBackground: In the adult hippocampus new neurons are continuously generated from neural stem cells (NSCs) present at the subgranular zone of the dentate gyrus. This process is controlled by Wnt signaling, which plays a complex role in regulating multiple steps of neurogenesis including maintenance, proliferation and differentiation of progenitor cells and the development of newborn neurons. Differential effects of Wnt signaling during progression of neurogenesis could be mediated by cell-type specific expression of Wnt receptors. Here we studied the potential role of Frizzled-1 (FZD1) receptor in adult hippocampal neurogenesis. Results: In the adult dentate gyrus, we determined that FZD1 is highly expressed in NSCs, neural progenitors and immature neurons. Accordingly, FZD1 is expressed in cultured adult hippocampal progenitors isolated from mouse brain. To evaluate the role of this receptor in vivo we targeted FZD1 in newborn cells using retroviral-mediated RNA interference. FZD1 knockdown resulted in a marked decrease in the differentiation of newborn cells into neurons and increased the generation of astrocytes, suggesting a regulatory role for the receptor in cell fate commitment. In addition, FZD1 knockdown induced an extended migration of adult-born neurons within the granule cell layer. However, no differences were observed in total dendritic length and dendritic arbor complexity between control and FZD1-deficient newborn neurons. Conclusions: Our results show that FZD1 regulates specific stages of adult hippocampal neurogenesis, being required for neuronal differentiation and positioning of newborn neurons into the granule cell layer, but not for morphological development of adult-born granule neurons.es_CL
dc.description.urihttps://molecularbrain.biomedcentral.com/articles/10.1186/s13041-016-0209-3
dc.identifier.citationMolecular Brain. Volume 9, Issue 1, 15 March 2016, Article number 209es_CL
dc.identifier.issn1756-6606
dc.identifier.otherDOI: 10.1186/s13041-016-0209-3
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/3978
dc.language.isoenes_CL
dc.publisherBIOMED CENTRALes_CL
dc.subjectAdult neurogenesises_CL
dc.subjectHippocampuses_CL
dc.subjectNeural progenitor cellses_CL
dc.subjectNeuronal differentiationes_CL
dc.subjectWnt signalinges_CL
dc.subjectFrizzledes_CL
dc.titleFrizzled-1 receptor regulates adult hippocampal neurogenesises_CL
dc.typeArtículoes_CL
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