Identification of a gene cluster for the formation of extracellular polysaccharide precursors in the chemolithoautotroph Acidithiobacillus ferrooxidans

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dc.contributor.authorBarreto, M
dc.contributor.authorJedlicki, E
dc.contributor.authorHolmes, DS
dc.date.accessioned2024-07-03T20:26:35Z
dc.date.available2024-07-03T20:26:35Z
dc.date.issued2005-06-01
dc.descriptionINDEXACIÓN: WEB OF SCIENCE.
dc.description.abstractA cluster of five genes, proposed to be involved in the formation of extracellular polysaccharide (EPS) precursors via the Leloir pathway, have been identified in the acidophilic autotroph Acidithiobacillus ferrooxidans. The order of the genes is luxA-galE-galK-pgm-galM, encoding a LuxA-like protein, UDP-glucose 4-epimerase, galactokinase, phosphoglucomutase, and galactose mutarotase, respectively. The gal cluster forms a single transcriptional unit and is therefore an operon. Two other putative genes of the Leloir pathway, galU, potentially encoding UDP-glucose pyrophosphorylase, and a gene designated galT-like, which may encode a gallactose-1-phosphate uridylyltransferase-like activity, were found unlinked in the genome. Using semiquantitative reverse transcription-PCR, the genes of the gal operon were shown to be expressed more during growth in iron medium than in growth in sulfur medium. The functions of galE, pgm, galU, and the galT-like gene were validated by complementation of Escherichia coli mutants and by in vitro enzyme assays. The data suggest that A. ferrooxidans is capable of synthesizing the EPS precursors UDP-glucose and UDP-galactose. In addition, genes rfbA, -B, -C, and -D were identified in the genome of A. ferrooxidans, suggesting that it can also synthesize the EPS precursor dTDP-rhamnose. Since EPSs constitute the major bulk of biofilms, this study may provide an initial model for the metabolic pathways involved in biofilm formation in A. ferrooxidans and aid in understanding the role of biofilms in mineral leaching and the formation of acid mine drainage.
dc.identifier.citationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY, Volume 71, Issue 6, Page 2902-2909
dc.identifier.doi10.1128/AEM.71.6.2902-2909.2005
dc.identifier.issn0099-2240
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/58183
dc.language.isoen
dc.publisherAMER SOC MICROBIOLOGY
dc.rights.licenseCC BY 4.0 ATRIBUCIÓN 4.0 INTERNACIONAL Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectCOLI GALACTOSE OPERON
dc.subjectTHIOBACILLUS-FERROOXIDANS
dc.subjectESCHERICHIA-COLI
dc.subjectEXOPOLYSACCHARIDE PRODUCTION
dc.subjectSTREPTOCOCCUS-THERMOPHILUS
dc.subjectF-420-DEPENDENT GLUCOSE-6-PHOSPHATE-DEHYDROGENASE
dc.subjectBIOSYNTHESIS
dc.subjectPYROPHOSPHORYLASE
dc.subjectMYCOBACTERIUM
dc.subjectEXPRESSION
dc.titleIdentification of a gene cluster for the formation of extracellular polysaccharide precursors in the chemolithoautotroph Acidithiobacillus ferrooxidans
dc.typeArtículo
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