DNA methylation and small interference RNAs participate in the regulation of MADS-box genes involved in dormancy in sweet cherry (Prunus avium L.)

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dc.contributor.authorRothkegel, Karin
dc.contributor.authorSánchez, Evelyn
dc.contributor.authorMontes, Christian
dc.contributor.authorGreve, Macarena
dc.contributor.authorTapia, Sebastián
dc.contributor.authorBravo, Soraya
dc.contributor.authorPrieto, Humberto
dc.contributor.authorAlmeida, Andréa Miyasaka
dc.date.accessioned2023-10-23T15:28:11Z
dc.date.available2023-10-23T15:28:11Z
dc.date.issued2017-12
dc.descriptionIndexación: Scopuses
dc.description.abstractEpigenetic modifications can yield information about connections between genotype, phenotype variation and environmental conditions. Bud dormancy release in temperate perennial fruit trees depends on internal and environmental signals such as cold accumulation and photoperiod. Previous investigations have noted the participation of epigenetic mechanisms in the control of this physiological process. We examined whether epigenetic modifications were modulated in MADS-box genes, potential candidates for the regulation of bud dormancy and flowering in sweet cherry (Prunus avium L.). We identified and cloned two MADS-box genes homologous to the already-characterized dormancy regulators DORMANCY-ASSOCIATED MADS-box (DAM3 and DAM5) from Prunus persica (L.) Batsch. Bisulfite sequencing of the identified genes (PavMADS1 and PavMADS2), Methylated DNA Immunoprecipitation and small RNA deep sequencing were performed to analyze the presence of DNA methylations that could be guided by non-coding RNAs in the floral buds exposed to differential chilling hours. The results obtained reveal an increase in the level of DNA methylation and abundance of matching small interference RNAs (siRNAs) in the promoter of PavMADS1 when the chilling requirement is complete. For the first intron and 5′ UTR of PavMADS1, de novo DNA methylation could be associated with the increase in the abundance of 24-nt siRNA matching the promoter area. Also, in the second large intron of PavMADS1, maintenance DNA methylation in all cytosine contexts is associated with the presence of homologous siRNAs in that zone. For PavMADS2, only maintenance methylation was present in the CG context, and no matching siRNAs were detected. Silencing of PavMADS1 and PavMADS2 coincided with an increase in Flowering Locus T expression during dormancy. In conclusion, DNA methylations and siRNAs appear to be involved in the silencing of PavMADS1 during cold accumulation and dormancy release in sweet cherry. © The Author 2017. Published by Oxford University Press. All rights reserved.es
dc.description.urihttps://academic-oup-com.recursosbiblioteca.unab.cl/treephys/article/37/12/1739/3852114
dc.identifier.citationTree Physiology Volume 37, Issue 12, Pages 1739 - 17511 December 2017es
dc.identifier.doi10.1093/treephys/tpx055
dc.identifier.issn0829-318X
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/53553
dc.language.isoenes
dc.publisherOxford University Presses
dc.rights.licenseCC BY 4.0 DEED
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectchilling requirementes
dc.subjectDAM geneses
dc.subjectepigenetic modificationses
dc.subjectfloral bud; floweringes
dc.subjectsmall RNAes
dc.titleDNA methylation and small interference RNAs participate in the regulation of MADS-box genes involved in dormancy in sweet cherry (Prunus avium L.)es
dc.typeArtículoes
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