Neuropathic Pain Induces Interleukin-1β Sensitive Bimodal Glycinergic Activity in the Central Amygdala

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dc.contributor.authorOliva, Carolina A.
dc.contributor.authorStehberg, Jimmy
dc.contributor.authorBarra, Rafael
dc.contributor.authorMariqueo, Trinidad
dc.date.accessioned2023-11-24T15:16:11Z
dc.date.available2023-11-24T15:16:11Z
dc.date.issued2022-07-01
dc.descriptionIndexación: Scopus.es
dc.description.abstractNeuropathic pain reduces GABA and glycine receptor (GlyR)-mediated activity in spinal and supraspinal regions associated with pain processing. Interleukin-1β (IL-1β) alters Central Amygdala (CeA) excitability by reducing glycinergic inhibition in a mechanism that involves the auxiliary β-subunit of GlyR (βGlyR), which is highly expressed in this region. However, GlyR activity and its modulation by IL-1β in supraspinal brain regions under neuropathic pain have not been studied. We performed chronic constriction injury (CCI) of the sciatic nerve in male Sprague Dawley rats, a procedure that induces hind paw plantar hyperalgesia and neuropathic pain. Ten days later, the rats were euthanized, and their brains were sliced. Glycinergic spontaneous inhibitory currents (sIPSCs) were recorded in the CeA slices. The sIPSCs from CeA neurons of CCI animals show a bimodal amplitude distribution, different from the normal distribution in Sham animals, with small and large amplitudes of similar decay constants. The perfusion of IL-1β (10 ng/mL) in these slices reduced the amplitudes within the first five minutes, with a pronounced effect on the largest amplitudes. Our data support a possible role for CeA GlyRs in pain processing and in the neuroimmune modulation of pain perception. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.es
dc.description.urihttps://www.mdpi.com/1422-0067/23/13/7356
dc.identifier.citationInternational Journal of Molecular Sciences, Volume 23, Issue 13, July-1 2022, Article number 7356es
dc.identifier.doi10.3390/ijms23137356
dc.identifier.issn1661-6596
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/54049
dc.language.isoenes
dc.publisherMDPIes
dc.rights.licenseCC BY 4.0 DEED Atribución 4.0 Internacional
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectCCIes
dc.subjectGlycine receptorses
dc.subjectNeuropathic paines
dc.subjectSpontaneous inhibitory currentses
dc.titleNeuropathic Pain Induces Interleukin-1β Sensitive Bimodal Glycinergic Activity in the Central Amygdalaes
dc.typeArtículoes
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