Chronic stress inhibits growth and induces proteolytic mechanisms through two different nonoverlapping pathways in the skeletal muscle of a teleost fish
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Archivos
Fecha
2018-06
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
American Physiological Society
Nombre de Curso
Licencia CC
CC BY 4.0
Licencia CC
Resumen
Chronic stress detrimen-tally affects animal health and homeostasis, with somatic growth, and thus skeletal muscle, being particularly affected. A detailed understanding of the underlying endocrine and molecular mechanisms of how chronic stress affects skeletal muscle growth remains lacking. To address this issue, the present study assessed primary (plasma corti-sol), secondary (key components of the GH/IGF system, muscular proteolytic pathways, and apoptosis), and tertiary (growth perfor-mance) stress responses in fine flounder (Paralichthys adspersus) exposed to crowding chronic stress. Levels of plasma cortisol, glucocorticoid receptor 2 (gr2), and its target genes (klf15 and redd1) mRNA increased significantly only at 4 wk of crowding (P < 0.05). The components of the GH/IGF system, including ligands, receptors, and their signaling pathways, were significantly downregulated at 7 wk of crowding (P < 0.05). Interestingly, chronic stress upregulated the ubiquitin-proteasome pathway and the intrinsic apoptosis pathways at 4wk (P < 0.01), whereas autophagy was only significantly activated at 7 wk (P < 0.05), and meanwhile the ubiquitin-proteasome and the apoptosis pathways returned to control levels. Overall growth was inhibited in fish in the 7-wk chronic stress trial (P < 0.05). In conclusion, chronic stress directly affects muscle growth and down-regulates the GH/IGF system, an action through which muscular catabolic mechanisms are promoted by two different and nonoverlapping proteolytic pathways. These findings provide new information on molecular mechanisms involved in the negative effects that chronic stress has on muscle anabolic/catabolic signaling balance. © 2018 American Physiological Society. All rights reserved.
Notas
Indexación Scopus
Palabras clave
Growth Hormone, Fish, Somatomedins, Apoptosis, Autophagy, Muscle growth, Stress, Ubiquitin-proteasome
Citación
American Journal of Physiology - Regulatory Integrative and Comparative Physiology Volume 314, Issue 1, Pages R102 - R113 January 2018
DOI
10.1152/ajpregu.00009.2017