Biomimetics: From bioinformatics to rational design of dendrimers as gene carriers

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dc.contributor.authorMárquez-Miranda, Valeria
dc.contributor.authorCamarada, María Belén
dc.contributor.authorAraya-Durán, Ingrid
dc.contributor.authorVaras-Concha, Ignacio
dc.contributor.authorAlmonacid, Daniel Eduardo
dc.contributor.authorGonzález-Nilo, Fernando Danilo
dc.date.accessioned2023-03-13T14:22:03Z
dc.date.available2023-03-13T14:22:03Z
dc.date.issued2015-09
dc.descriptionIndexación: Scopuses
dc.description.abstractBiomimetics, or the use of principles of Nature for developing new materials, is a paradigm that could help Nanomedicine tremendously. One of the current challenges in Nanomedicine is the rational design of new efficient and safer gene carriers. Poly(amidoamine) (PAMAM) dendrimers are a well-known class of nanoparticles, extensively used as nonviral nucleic acid carriers, due to their positively charged end-groups. Yet, there are still several aspects that can be improved for their successful application in in vitro and in vivo systems, including their affinity for nucleic acids as well as lowering their cytotoxicity. In the search of new functional groups that could be used as new dendrimer-reactive groups, we followed a biomimetic approach to determine the amino acids with highest prevalence in protein-DNA interactions. Then we introduced them individually as terminal groups of dendrimers, generating a new class of nanoparticles. Molecular dynamics studies of two systems: PAMAM-Arg and PAMAM-Lys were also performed in order to describe the formation of complexes with DNA. Results confirmed that the introduction of amino acids as terminal groups in a dendrimer increases their affinity for DNA and the interactions in the complexes were characterized at atomic level. We end up by briefly discussing additional modifications that can be made to PAMAM dendrimers to turned them into promising new gene carriers. Copyright: © 2015 Márquez-Miranda et al.es
dc.description.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0138392
dc.identifier.doi10.1371/journal.pone.0138392
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/47488
dc.language.isoenes
dc.publisherPublic Library of Sciencees
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectPolyamidoaminees
dc.subjectDrug Delivery Systemses
dc.subjectDendrimerses
dc.titleBiomimetics: From bioinformatics to rational design of dendrimers as gene carrierses
dc.typeArtículoes
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PLoS ONE Volume 10, Issue 918 September 2015 Article number A1479
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