New insight into the biological activity of Salmo salar NK-lysin antimicrobial peptides

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dc.contributor.authorOrtega, Leonardo
dc.contributor.authorCarrera, Crisleri
dc.contributor.authorMuñoz-Flores, Carolina
dc.contributor.authorSalazar, Santiago
dc.contributor.authorVillegas, Milton F.
dc.contributor.authorStarck, María F.
dc.contributor.authorValenzuela, Ariel
dc.contributor.authorAgurto, Niza
dc.contributor.authorMontesino, Raquel
dc.contributor.authorAstuya, Allisson
dc.contributor.authorParra, Natalie
dc.contributor.authorPérez, Ercilia T.
dc.contributor.authorSantibáñez, Natacha
dc.contributor.authorRomero, Alex
dc.contributor.authorRuíz, Pamela
dc.contributor.authorLamazares, Emilio
dc.contributor.authorReyes, Fátima
dc.contributor.authorSánchez, Oliberto
dc.contributor.authorToledo, Jorge R.
dc.contributor.authorAcosta, Jannel
dc.date.accessioned2024-05-07T20:45:02Z
dc.date.available2024-05-07T20:45:02Z
dc.date.issued2024-01
dc.description.abstractNK-lysin is a potent antimicrobial peptide (AMP) with antimicrobial activity against bacteria, fungi, viruses, and parasites. NK-lysin is a type of granulysin, a member of the saposin-like proteins family first isolated from a pig’s small intestine. In previous work, for the first time, we identified four variants of nk-lysin from Atlantic salmon (Salmo salar) using EST sequences. In the present study, we reported and characterized two additional transcripts of NK-lysin from S. salar. Besides, we evaluated the tissue distribution of three NK-lysins from S. salar and assessed the antimicrobial, hemolytic, and immunomodulatory activities and signaling pathways of three NK-lysin-derived peptides. The synthetic peptides displayed antimicrobial activity against Piscirickettsia salmonis (LF-89) and Flavobacterium psychrophilum. These peptides induced the expression of immune genes related to innate and adaptive immune responses in vitro and in vivo. The immunomodulatory activity of the peptides involves the mitogen-activated protein kinases-mediated signaling pathway, including p38, extracellular signal-regulated kinase 1/2, and/or c-Jun N-terminal kinases. Besides, the peptides modulated the immune response induced by pathogen-associated molecular patterns (PAMPs). Our findings show that NK-lysin could be a highly effective immunostimulant or vaccine adjuvant for use in fish aquaculture.
dc.description.urihttps://www-scopus-com.recursosbiblioteca.unab.cl/record/display.uri?eid=2-s2.0-85191069985&origin=resultslist&sort=plf-f&src=s&nlo=&nlr=&nls=&sid=d10da7a0e741967abc8eb8eb44c0421a&sot=aff&sdt=cl&cluster=scofreetoread%2c%22all%22%2ct&sl=61&s=AF-ID%28%22Universidad+Andr%c3%a9s+Bello%22+60002636%29+AND+SUBJAREA%28IMMU%29&relpos=12&citeCnt=0&searchTerm=
dc.identifier.citationFrontiers in Immunology Open Access Volume 152024 Article number 1191966
dc.identifier.doi10.3389/fimmu.2024.1191966
dc.identifier.issn16643224
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/56617
dc.language.isoen
dc.publisherFrontiers Media SA
dc.rights.licenseCC BY 4.0 DEED Attribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleNew insight into the biological activity of Salmo salar NK-lysin antimicrobial peptides
dc.typeArtículo
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