Global transcriptome profiles provide insights into muscle cell development and differentiation on microstructured marine biopolymer scaffolds for cultured meat production

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dc.contributor.authorBezjak, Dragica
dc.contributor.authorOrellana, Nicole
dc.contributor.authorValdivia, Guillermo
dc.contributor.authorAcevedo, Cristian A.
dc.contributor.authorValdes, Jorge H.
dc.date.accessioned2024-11-14T13:48:50Z
dc.date.available2024-11-14T13:48:50Z
dc.date.issued2024-12
dc.descriptionIndexación: Scopus.
dc.description.abstractBiomaterial scaffolds play a pivotal role in the advancement of cultured meat technology, facilitating essential processes like cell attachment, growth, specialization, and alignment. Currently, there exists limited knowledge concerning the creation of consumable scaffolds tailored for cultured meat applications. This investigation aimed to produce edible scaffolds featuring both smooth and patterned surfaces, utilizing biomaterials such as salmon gelatin, alginate, agarose and glycerol, pertinent to cultured meat and adhering to food safety protocols. The primary objective of this research was to uncover variations in transcriptomes profiles between flat and microstructured edible scaffolds fabricated from marine-derived biopolymers, leveraging high-throughput sequencing techniques. Expression analysis revealed noteworthy disparities in transcriptome profiles when comparing the flat and microstructured scaffold configurations against a control condition. Employing gene functional enrichment analysis for the microstructured versus flat scaffold conditions yielded substantial enrichment ratios, highlighting pertinent gene modules linked to the development of skeletal muscle. Notable functional aspects included filament sliding, muscle contraction, and the organization of sarcomeres. By shedding light on these intricate processes, this study offers insights into the fundamental mechanisms underpinning the generation of muscle-specific cultured meat. © The Author(s) 2024.
dc.description.urihttps://www-nature-com.recursosbiblioteca.unab.cl/articles/s41598-024-61458-9
dc.identifier.doi10.1038/s41598-024-61458-9
dc.identifier.issn2045-2322
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/61978
dc.language.isoen
dc.publisherScientific Reports, Volume 14, Issue 1 December 2024 Article number 10931
dc.rights.licenseAttribution 4.0 International CC BY 4.0 Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAlginates
dc.subjectAnimals
dc.subjectBiocompatible Materials
dc.subjectBiopolymers
dc.subjectCell Differentiation
dc.subjectGelatin
dc.subjectGene Expression Profiling
dc.subjectIn Vitro Meat
dc.subjectMeat
dc.subjectMuscle Cells
dc.subjectMuscle Development
dc.subjectSalmon
dc.subjectSepharose
dc.subjectTissue Scaffolds
dc.subjectTranscriptome
dc.titleGlobal transcriptome profiles provide insights into muscle cell development and differentiation on microstructured marine biopolymer scaffolds for cultured meat production
dc.typeArtículo
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