Subtyping of clostridium difficile PCR ribotypes 591, 106 and 002, the dominant strain types circulating in Medellin, Colombia

Vista sencilla de ítem
dc.contributor.authorSalazar, C.L.
dc.contributor.authorReyes, C.
dc.contributor.authorCienfuegos-Gallet, A.V.
dc.contributor.authorBest, E.
dc.contributor.authorAtehortua, S.
dc.contributor.authorSierra, P.
dc.contributor.authorCorrea, M.M.
dc.date.accessioned2019-04-22T16:12:09Z
dc.date.available2019-04-22T16:12:09Z
dc.date.issued2018-04
dc.descriptionIndexación: Scopus.es_ES
dc.descriptionDepartamento Administrativo de Ciencia, Tecnología e Innovación 1115569344410 COLCIENCIAS (Colombia)
dc.descriptionFondo Nacional de Ciencia y Tecnología de Chile (FONDECYT Grant1151025)
dc.description.abstractWe aimed to achieve a higher typing resolution within the three dominant Clostridium difficile ribotypes (591,106 and 002) circulating in Colombia. A total of 50 C. difficile isolates we had previously typed by PCR-ribotyping, representing the major three ribotypes circulating in Colombia, were analyzed. Twenty-seven isolates of ribotype 591, 12 of ribotype 106 and 11 of ribotype 002 were subtyped by multiple locus variable-number tandem-repeat analysis (MLVA). The presence of the PaLoc genes (tcdA/tcdB), toxin production in culture and antimicrobial susceptibility were also determined. From the total C. difficile ribotypes analyzed, 20 isolates (74%) of ribotype 591, nine (75%) of ribotype 106 and five (45.5%) of ribotype 002 were recovered from patients with Clostridium difficile infection (CDI). MLVA allowed us to recognize four and two different clonal complexes for ribotypes 591 and 002, respectively, having a summed tandem-repeat difference (STRD) <2, whereas none of the ribotype 106 isolates were grouped in a cluster or clonal complex having a STRD >10. Six ribotype 591 and three ribotype 002 isolates belonging to a defined clonal complex were isolated on the same week in two different hospitals. All ribotypes harbored either tcdA+/tcdB+ or tcdA-/ tcdB+ PaLoc genes. Moreover, 94% of the isolates were positive for toxin in culture. All isolates were susceptible to vancomycin and metronidazole, while 75% to 100% of the isolates were resistant to clindamycin, and less than 14.8% of ribotype 591 isolates were resistant to moxifloxacina. No significant differences were found among ribotypes with respect to demographic and clinical patients’ data; however, our results demonstrated a high molecular heterogeneity of C. difficile strains circulating in Colombia. © 2018 Salazar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.es_ES
dc.description.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0195694
dc.identifier.citationPLoS ONE, 13(4), art. no. e0195694es_ES
dc.identifier.issn1932-6203
dc.identifier.other10.1371/journal.pone.0195694
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/8554
dc.language.isoenes_ES
dc.publisherPublic Library of Sciencees_ES
dc.subjectClindamycines_ES
dc.subjectClostridium difficile toxin Aes_ES
dc.subjectClostridium difficile toxin Bes_ES
dc.subjectMetronidazolemes_ES
dc.subjectOxifloxacines_ES
dc.subjectVancomycines_ES
dc.titleSubtyping of clostridium difficile PCR ribotypes 591, 106 and 002, the dominant strain types circulating in Medellin, Colombiaes_ES
dc.typeArtículoes_ES
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Salazar-2018-Subtyping-of-clostridium-difficile.pdf
Tamaño:
4.35 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLES
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista