Effect of single post-ovulatory administration of mifepristone (RU486) on transcript profile during the receptive period in human endometrium

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dc.contributor.authorCuevas, Catherina A.
dc.contributor.authorTapia-Pizarro, Alejandro
dc.contributor.authorSalvatierra, Ana María
dc.contributor.authorMunroe, David J.
dc.contributor.authorVelasquez, Luis
dc.contributor.authorCroxatto, Horacio B.
dc.date.accessioned2023-11-09T20:59:39Z
dc.date.available2023-11-09T20:59:39Z
dc.date.issued2016-04
dc.descriptionIndexación: Scopus.es
dc.description.abstractProgesterone regulates uterine function during the luteal phase and is essential for the acquisition of endometrial receptivity. The objective of the present study was to identify endometrial transcripts whose expression is altered during the window of implantation after the administration of 200 mg of the antiprogestin mifepristone, 48 h after the LH peak (LHC2, LHC0ZLH peak), and to determine the relationship of these transcripts with those regulated during the acquisition of receptivity. Endometrial samples were obtained in LHC7 from seven women of proven fertility, each one contributing with one cycle treated with placebo and another with mifepristone. Additionally, endometrial samples were obtained in LHC2 and LHC7 during a single untreated spontaneous cycle from seven normal fertile women as a reference. DNA microarrays were used to identify transcripts significantly regulated (defined as R2.0-fold change with false discovery rate below 1% using t-test) with the administration of mifepristone vs placebo, or during the transition from pre-receptive to receptive (LHC2 vs LHC7). Approximately 2000 transcripts were significantly regulated in both comparisons (mifepristone vs placebo and LHC2 vs LHC7), but only 777 of them were coincident and displayed opposite regulation except for 25. The mRNA level for eight selected genes regulated by mifepristone was confirmed by real-time RT-PCR. We conclude that not all changes in endometrial transcript levels occurring in the transition from LHC2 to LHC7 seem to be regulated by the progesterone receptor and w37% of the genes whose transcript levels changed by effect of mifepristone could be associated with the acquisition of receptivity. Reproduction (2016) 151 331–349es
dc.description.urihttps://rep.bioscientifica.com/view/journals/rep/151/4/331.xml?body=pdf-59407
dc.identifier.citationReproduction. Volume 151, Issue 4, Pages 331 - 349. April 2016es
dc.identifier.doi10.1530/REP-15-0458
dc.identifier.issn1470-1626
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/53884
dc.language.isoenes
dc.publisherBioScientifica Ltd.es
dc.subjectEmbryologyes
dc.subjectReproductive Medicinees
dc.subjectEndocrinologyes
dc.subjectObstetrics and Gynecologyes
dc.subjectCell Biologyes
dc.titleEffect of single post-ovulatory administration of mifepristone (RU486) on transcript profile during the receptive period in human endometriumes
dc.typeArtículoes
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