β1-subunit-induced structural rearrangements of the Ca2+- and voltage-activated K+ (BK) channel

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dc.contributor.authorCastillo, Juan P.
dc.contributor.authorSánchez-Rodríguez, Jorge E.
dc.contributor.authorHyde, H. Clark
dc.contributor.authorZaelzer, Cristian A.
dc.contributor.authorAguayo, Daniel
dc.contributor.authorSepúlveda, Romina V.
dc.contributor.authorLuk, Louis Y.P.
dc.contributor.authorKent, Stephen B.H.
dc.contributor.authorGonzalez-Nilo, Fernando D.
dc.contributor.authorBezanilla, Francisco
dc.contributor.authorLatorre, Ramón
dc.date.accessioned2023-10-25T20:02:11Z
dc.date.available2023-10-25T20:02:11Z
dc.date.issued2016-06
dc.descriptionIndexación: Scopus.es
dc.description.abstractLarge-conductance Ca2+- and voltage-activated K+ (BK) channels are involved in a large variety of physiological processes. Regulatory β-subunits are one of the mechanisms responsible for creating BK channel diversity fundamental to the adequate function of many tissues. However, little is known about the structure of its voltage sensor domain. Here, we present the external architectural details of BK channels using lanthanide-based resonance energy transfer (LRET). We used a genetically encoded lanthanide-binding tag (LBT) to bind terbium as a LRET donor and a fluorophore-labeled iberiotoxin as the LRET acceptor for measurements of distances within the BK channel structure in a living cell. By introducing LBTs in the extracellular region of the α- or β1-subunit, we determined (i) a basic extracellular map of the BK channel, (ii) β1-subunit–induced rearrangements of the voltage sensor in α-subunits, and (iii) the relative position of the β1-subunit within the α/β1-subunit complex.es
dc.description.urihttps://www.pnas.org/doi/full/10.1073/pnas.1606381113
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America. Volume 113, Issue 23, Pages E3231 - E3239. 7 June 2016es
dc.identifier.doi10.1073/pnas.1606381113
dc.identifier.issn0027-8424
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/53625
dc.language.isoenes
dc.publisherNational Academy of Scienceses
dc.subjectLanthanide Resonance Energy Transferes
dc.subjectBK Channelses
dc.subjectβ1-subunites
dc.titleβ1-subunit-induced structural rearrangements of the Ca2+- and voltage-activated K+ (BK) channeles
dc.typeArtículoes
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