UUAT1 is a golgi-localized UDP-uronic acid transporter that modulates the polysaccharide composition of arabidopsis seed mucilage

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dc.contributor.authorSaez-Aguayo, Susana
dc.contributor.authorRautengarten, Carsten
dc.contributor.authorTemple, Henry
dc.contributor.authorSanhueza, Dayan
dc.contributor.authorEjsmentewicz, Troy
dc.contributor.authorSandoval-Ibañez, Omar
dc.contributor.authorDoñas, Daniela
dc.contributor.authorParra-Rojas, Juan Pablo
dc.contributor.authorEbert, Berit
dc.contributor.authorLehner, Arnaud
dc.contributor.authorMollet, Jean-Claude
dc.contributor.authorDupree, Paul
dc.contributor.authorScheller, Henrik V.
dc.contributor.authorHeazlewood, Joshua L.
dc.contributor.authorReyes, Francisca C.
dc.contributor.authorOrellana, Ariel
dc.date.accessioned2024-09-23T17:09:40Z
dc.date.available2024-09-23T17:09:40Z
dc.date.issued2017
dc.descriptionIndexación: Scopus
dc.description.abstractUDP-glucuronic acid (UDP-GlcA) is the precursor of many plant cell wall polysaccharides and is required for production of seed mucilage. Following synthesis in the cytosol, it is transported into the lumen of the Golgi apparatus, where it is converted to UDP-galacturonic acid (UDP-GalA), UDP-arabinose, and UDP-xylose. To identify the Golgi-localized UDP-GlcA transporter, we screened Arabidopsis thaliana mutants in genes coding for putative nucleotide sugar transporters for altered seed mucilage, a structure rich in the GalA-containing polysaccharide rhamnogalacturonan I. As a result, we identified UUAT1, which encodes a Golgi-localized protein that transports UDP-GlcA and UDP-GalA in vitro. The seed coat of uuat1 mutants had less GalA, rhamnose, and xylose in the soluble mucilage, and the distal cell walls had decreased arabinan content. Cell walls of other organs and cells had lower arabinose levels in roots and pollen tubes, but no differences were observed in GalA or xylose contents. Furthermore, the GlcA content of glucuronoxylan in the stem was not affected in the mutant. Interestingly, the degree of homogalacturonan methylation increased in uuat1. These results suggest that this UDP-GlcA transporter plays a key role defining the seed mucilage sugar composition and that its absence produces pleiotropic effects in this component of the plant extracellular matrix. © 2016 American Society of Plant Biologists.
dc.description.urihttps://academic-oup-com.recursosbiblioteca.unab.cl/plcell/article/29/1/129/6099017
dc.identifier.citationPlant Cell Volume 29, Issue 1, Pages 129 - 143January 2017
dc.identifier.doi10.1105/tpc.16.00465
dc.identifier.issn1040-4651
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/60432
dc.language.isoen
dc.publisherAmerican Society of Plant Biologists
dc.rights.licenseAtribución/Reconocimiento 4.0 Internacional CC BY 4.0 Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.titleUUAT1 is a golgi-localized UDP-uronic acid transporter that modulates the polysaccharide composition of arabidopsis seed mucilage
dc.typeArtículo
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