Participation of two sRNA RyhB homologs from the fish pathogen Yersinia ruckeri in bacterial physiology

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dc.contributor.authorAcuña, Lillian G.
dc.contributor.authorBarros, M. José
dc.contributor.authorMontt, Fernanda
dc.contributor.authorPeñaloza, Diego
dc.contributor.authorNúñez, Paula
dc.contributor.authorValdés, Iván
dc.contributor.authorGil, Fernando
dc.contributor.authorFuentes, Juan A.
dc.contributor.authorCalderón, Iván L.
dc.date.accessioned2025-01-27T20:04:56Z
dc.date.available2025-01-27T20:04:56Z
dc.date.issued2021
dc.descriptionIndexación: Scopus.
dc.description.abstractSmall noncoding RNAs (sRNAs) are important regulators of gene expression and physiology in bacteria. RyhB is an iron-responsive sRNA well characterized in Escherichia coli and conserved in other Enterobacteriaceae. In this study, we identified and characterized two RyhB homologs (named RyhB-1 and RyhB-2) in the fish pathogen Yersinia ruckeri. We found that, as in other Enterobacteriaceae, both RyhB-1 and RyhB-2 are induced under iron starvation, repressed by the Fur regulator, and depend on Hfq for stability. Despite these similarities in expression, the mutant strains of Y. ruckeri lacking RyhB-1 (ΔryhB-1) or RyhB-2 (ΔryhB-2) exhibited differential phenotypes. In comparison with the wild type, the ΔryhB-1 strain showed a hypermotile phenotype, reduced biofilm formation, increased replication rate, faster growth, and increased ATP levels in bacterial cultures. By contrast, in salmon cell cultures, the ΔryhB-1 strain exhibited an increased survival. On the other hand, the ΔryhB-2 strain was non-motile and showed augmented biofilm formation as compared to the wild type. The expression of a subset of RyhB conserved targets, selected from different bacterial species, was analyzed by quantitative RT-PCR in wild type, ΔryhB-1, ΔryhB-2, and ΔryhB-1 ΔryhB-2 strains cultured in iron-depleted media. RyhB-1 negatively affected the expression of most analyzed genes (sodB, acnA, sdhC, bfr, fliF, among others), whose functions are related to metabolism and motility, involving iron-containing proteins. Among the genes analyzed, only sdhC and bfr appeared as targets for RyhB-2. Taken together, these results indicate that Y. ruckeri RyhB homologs participate in the modulation of the bacterial physiology with non-redundant roles. © 2020 Elsevier GmbH
dc.description.urihttps://www.sciencedirect.com/science/article/pii/S0944501320304973?pes=vor&utm_source=scopus&getft_integrator=scopus
dc.identifier.doi10.1016/j.micres.2020.126629
dc.identifier.issn09445013
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/63311
dc.language.isoen_US
dc.publisherMicrobiological Research, Volume 242January 2021 Article number 126629
dc.rights.licenseAtribución/Reconocimiento 4.0 Internacional CC BY 4.0 Código legal
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/legalcode.es
dc.subjectAnimals
dc.subjectBacterial Physiological Phenomena
dc.subjectBacterial Proteins
dc.subjectBiofilms
dc.subjectEscherichia coli
dc.subjectFish Diseases
dc.subjectFishes
dc.subjectGene Deletion
dc.subjectGene Expression Regulation
dc.subjectBacterial
dc.subjectHomeostasis
dc.subjectIron
dc.subjectRNA
dc.subjectBacterial
dc.subjectSmall Untranslated
dc.subjectYersinia Infections
dc.subjectrucker
dc.subjectPhenotype
dc.titleParticipation of two sRNA RyhB homologs from the fish pathogen Yersinia ruckeri in bacterial physiology
dc.typeArtículo
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