Transcriptomic analysis of the hepatic response to stress in the red cusk-eel (Genypterus chilensis): Insights into lipid metabolism, oxidative stress and liver steatosis

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dc.contributor.authorNaour, S.
dc.contributor.authorEspinoza, B.M.
dc.contributor.authorAedo, J.E.
dc.contributor.authorZuloaga, R.
dc.contributor.authorMaldonado, J.
dc.contributor.authorBastias-Molina, M.
dc.contributor.authorSilva, H.
dc.contributor.authorMeneses, C.
dc.contributor.authorGallardo-Escarate, C.
dc.contributor.authorMolina, A.
dc.contributor.authorValdés, J.A.
dc.date.accessioned2017-10-26T16:51:13Z
dc.date.available2017-10-26T16:51:13Z
dc.date.issued2017-04
dc.descriptionIndexación: Scopus.es_CL
dc.description.abstractTeleosts exhibit a broad divergence in their adaptive response to stress, depending on the magnitude, duration, and frequency of stressors and the species receiving the stimulus. We have previously reported that the red cusk-eel (Genypterus chilensis), an important marine farmed fish, shows a physiological response to stress that results in increased skeletal muscle atrophy mediated by over-expression of components of the ubiquitin proteasome and autophagy-lysosomal systems. To better understand the systemic effects of stress on the red cusk-eel metabolism, the present study assessed the transcriptomic hepatic response to repetitive handling-stress. Using high-Throughput RNA-seq, 259 up-regulated transcripts were found, mostly associated with angiogenesis, gluconeogenesis, and triacylglyceride catabolism. Conversely, 293 transcripts were down-regulated, associated to cholesterol biosynthesis, PPARα signaling, fatty acid biosynthesis, and glycolysis. This gene signature was concordant with hepatic metabolite levels and hepatic oxidative damage. Moreover, the increased plasmatic levels of AST (aspartate aminotransferase), ALT (alanine aminotransferase) and AP (alkaline phosphatase), as well as liver histology suggest stress-induced liver steatosis. This study offers an integrative molecular and biochemical analysis of the hepatic response to handling-stress, and reveals unknown aspects of lipid metabolism in a non-model teleost.es_CL
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0176447
dc.identifier.citationPLoS ONE. Volume 12, Issue 4, April 2017, Article number e0176447es_CL
dc.identifier.issn1932-6203
dc.identifier.otherDOI: 10.1371/journal.pone.0176447
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/4480
dc.language.isoenes_CL
dc.publisherPublic Library of Sciencees_CL
dc.subjectAnimales_CL
dc.subjectFishes_CL
dc.subjectGene expression profilinges_CL
dc.subjectGene ontologyes_CL
dc.subjectGeneticses_CL
dc.subjectLipid metabolismes_CL
dc.subjectLiveres_CL
dc.subjectMetabolismes_CL
dc.subjectNonalcoholic fatty liveres_CL
dc.subjectOxidative stresses_CL
dc.subjectSequence analysises_CL
dc.titleTranscriptomic analysis of the hepatic response to stress in the red cusk-eel (Genypterus chilensis): Insights into lipid metabolism, oxidative stress and liver steatosises_CL
dc.typeArtículoes_CL
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