Epigenetic control of the bone-master Runx2 gene during osteoblast-lineage commitment by the histone demethylase JARID1B/KDM5B

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dc.contributor.authorRojas, Adriana
dc.contributor.authorAguilar, Rodrigo
dc.contributor.authorHenriquez, Berta
dc.contributor.authorLian, Jane B.
dc.contributor.authorStein, Janet L.
dc.contributor.authorStein, Gary S.
dc.contributor.authorVan Wijnen, Andre J.
dc.contributor.authorVan Zundert, Brigitte
dc.contributor.authorAllende, Miguel L.
dc.contributor.authorMontecino, Martin
dc.date.accessioned2023-02-15T13:40:42Z
dc.date.available2023-02-15T13:40:42Z
dc.date.issued2015-12
dc.descriptionIndexación: Scopuses
dc.description.abstractTranscription factor Runx2 controls bone development and osteoblast differentiation by regulating expression of a significant number of bone-related target genes. Here, we report that transcriptional activation and repression of the Runx2 gene via its osteoblast-specific P1 promoter (encoding mRNA for the Runx2/p57 isoform) is accompanied by selective deposition and elimination of histone marks during differentiation of mesenchymal cells to the osteogenic and myoblastic lineages. These epigenetic profiles are mediated by key components of the Trithorax/COMPASS-like and Polycomb group complexes together with histone arginine methylases like PRMT5 and lysine demethylases like JARID1B/KDM5B. Importantly, knockdown of the H3K4me2/3 demethylase JARID1B, but not of the demethylases UTX and NO66, prevents repression of the Runx2 P1 promoter during myogenic differentiation of mesenchymal cells. The epigenetically forced expression of Runx2/p57 and osteocalcin, a classical bone-related target gene, under myoblastic-differentiation is accompanied by enrichment of the H3K4me3 and H3K27ac marks at the Runx2 P1 promoter region. Our results identify JARID1B as a key component of a potent epigenetic switch that controls mesenchymal cell fate into myogenic and osteogenic lineages. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.es
dc.description.urihttps://www-sciencedirect-com.recursosbiblioteca.unab.cl/science/article/pii/S0021925820450033?via%3Dihub
dc.identifier.doi10.1074/jbc.M115.657825
dc.identifier.issn0021-9258
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/46870
dc.language.isoenes
dc.publisherAmerican Society for Biochemistry and Molecular Biology Inc.es
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectCleidocranial Dysplasiaes
dc.subjectOsteoblastses
dc.subjectTranscription Factor RUNX2es
dc.titleEpigenetic control of the bone-master Runx2 gene during osteoblast-lineage commitment by the histone demethylase JARID1B/KDM5Bes
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Journal of Biological Chemistry Volume 290, Issue 47, Pages 28329 - 2834220 November 2015
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