Development of a PHBV nanoparticle as a peptide vehicle for NOD1 activation

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dc.contributor.authorCabaña-Brunod, Mauricio
dc.contributor.authorHerrera, Pablo A.
dc.contributor.authorMárquez-Miranda, Valeria
dc.contributor.authorLlancalahuen, Felipe M.
dc.contributor.authorDuarte, Yorley
dc.contributor.authorGonzález-Nilo, Danilo
dc.contributor.authorFuentes, Juan A.
dc.contributor.authorVilos, Cristián
dc.contributor.authorVelásquez, Luis
dc.contributor.authorOtero, Carolina
dc.date.accessioned2022-06-13T13:53:03Z
dc.date.available2022-06-13T13:53:03Z
dc.date.issued2021
dc.descriptionIndexación Scopuses
dc.description.abstractNOD1 is an intracellular receptor that, when activated, induces gene expression of pro-inflammatory factors promoting macrophages and neutrophils recruitment at the infection site. However, iE-DAP, the dipeptide agonist that promotes this receptor's activation, cannot permeate cell membranes. To develop a nanocarrier capable of achieving a high and prolonged activation over time, iE-DAP was encapsulated in nanoparticles (NPs) made of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). The physicochemical properties, colloidal stability, encapsulation efficiency, and cellular uptake of iE-DAP-loaded PHVB NPs were analyzed. Results evidenced that physicochemical properties of iE-DAP-loaded NPs remained stable over time, and NPs were efficiently internalized into cells, a process that depends on time and concentration. Moreover, our results showed that NPs elicited a controlled cargo release in vitro, and the encapsulated agonist response was higher than its free form, suggesting the possibility of activating intracellular receptors triggering an immune response through the release of NOD1 agonist.es
dc.identifier.citationDrug Delivery Volume 28, Issue 1, Pages 1020 - 10302021es
dc.identifier.doi10.1080/10717544.2021.1923862
dc.identifier.issn1071-7544
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/22779
dc.language.isoenes
dc.publisherTaylor and Francis Ltd.es
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectinnate immunityes
dc.subjectmacrophagees
dc.subjectNanoparticleses
dc.subjectNod1 agonistes
dc.subjectPHBVes
dc.titleDevelopment of a PHBV nanoparticle as a peptide vehicle for NOD1 activationes
dc.typeArtículoes
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