Modified intracellular-associated phenotypes in a recombinant Salmonella typhi expressing S. typhimurium SPI-3 sequences

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dc.contributor.authorRetamal, Patricio
dc.contributor.authorCastillo-Ruiz, Mario
dc.contributor.authorVillagra, Nicolás A.
dc.contributor.authorMorgado, Juan
dc.contributor.authorMora, Guido C.
dc.date.accessioned2024-04-09T15:03:18Z
dc.date.available2024-04-09T15:03:18Z
dc.date.issued2010-02-24
dc.descriptionINDEXACIÓN: SCOPUS.
dc.description.abstractA bioinformatics comparison of Salmonella Pathogenicity Island 3 sequences from S. Typhi and S. Typhimurium serovars showed that ten genes are highly conserved. However three of them are pseudogenes in S. Typhi. Our aim was to understand what functions are lost in S. Typhi due to pseudogenes by constructing a S. Typhi genetic hybrid carrying the SPI-3 region of S. Typhimurium instead of its own SPI-3. We observed that under stressful conditions the hybrid strain showed a clear impairment in resistance to hydrogen peroxide and decreased survival within U937 culture monocytes. We hypothesized that the marT-fidL operon, encoded in SPI-3, was responsible for the new phenotypes because marT is a pseudogen in S. Typhi and has a demonstrated role as a transcriptional regulator in S. Typhimurium. Therefore we cloned and transferred the S. Typhimurium marT-fidL operon into S. Typhi and confirmed that invasion of monocytes was dramatically decreased. Finally, our findings suggest that the genomic and functional differences between SPI-3 sequences have implications in the host specificity of Typhi and Typhimurium serovars. Copyright: © 2010 Retamal et al.
dc.identifier.citationPLoS ONE, Volume 5, Issue 2, 24 February 2010, Article number e9394
dc.identifier.doi10.1371/journal.pone.0009394
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/55786
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.licenseCC BY 4.0 DEED Atribución 4.0 Internacional
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectAnaerobiosis
dc.subjectGene Expression Regulation, Bacterial
dc.subjectGene Order
dc.subjectGenes, Bacterial
dc.subjectGenomic Islands
dc.subjectGenotype
dc.subjectHumans
dc.subjectHydrogen Peroxide
dc.subjectHydrogen-Ion Concentration
dc.subjectMicrobial Viability
dc.subjectMutation
dc.subjectOperon
dc.subjectPhenotype
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSalmonella typhi
dc.subjectSalmonella typhimurium
dc.subjectTemperature
dc.subjectTemperature
dc.subjectTransformation, Genetic
dc.subjectU937 Cells
dc.titleModified intracellular-associated phenotypes in a recombinant Salmonella typhi expressing S. typhimurium SPI-3 sequences
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