Persistent oxytetracycline exposure induces an inflammatory process that improves regenerative capacity in zebrafish larvae

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dc.contributor.authorBarros-Becker, F.
dc.contributor.authorRomero, J.
dc.contributor.authorPulgar, A.
dc.contributor.authorFeijóo, C.
dc.date.accessioned2023-06-27T19:56:27Z
dc.date.available2023-06-27T19:56:27Z
dc.date.issued2012-03
dc.descriptionIndexación: Scopus.es
dc.description.abstractThe excessive use of antibiotics in aquaculture can adversely affect not only the environment, but also fish themselves. In this regard, there is evidence that some antibiotics can activate the immune system and reduce their effectiveness. None of those studies consider in detail the adverse inflammatory effect that the antibiotic remaining in the water may cause to the fish. In this work, we use the zebrafish to analyze quantitatively the effects of persistent exposure to oxytetracycline, the most common antibiotic used in fish farming. Methodology: We developed a quantitative assay in which we exposed zebrafish larvae to oxytetracycline for a period of 24 to 96 hrs. In order to determinate if the exposure causes any inflammation reaction, we evaluated neutrophils infiltration and quantified their total number analyzing the Tg(mpx:GFP)i114 transgenic line by fluorescence stereoscope, microscope and flow cytometry respectively. On the other hand, we characterized the process at a molecular level by analyzing several immune markers (il-1β, il-10, lysC, mpx, cyp1a) at different time points by qPCR. Finally, we evaluated the influence of the inflammation triggered by oxytetracycline on the regeneration capacity in the lateral line. Conclusions: Our results suggest that after 48 hours of exposure, the oxytetracycline triggered a widespread inflammation process that persisted until 96 hours of exposure. Interestingly, larvae that developed an inflammation process showed an improved regeneration capacity in the mechanosensory system lateral line.es
dc.description.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0036827
dc.identifier.citationPLoS ONE, Volume 7, Issue 510, May 2012, Article number e36827es
dc.identifier.doi10.1371/journal.pone.0036827
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/51094
dc.language.isoenes
dc.publisherPublic Library of Science (PLoS)es
dc.rights.licenseAttribution 2.0 Generic (CC BY 2.0)
dc.rights.urihttps://plos.org/open-science/open-access/
dc.subjectFlorfenicoles
dc.subjectEnrofloxacines
dc.subjectAnti-Bacterial Agentses
dc.subjectlysozymees
dc.subjectmyeloperoxidasees
dc.subjectoxytetracyclinees
dc.subjectantiinfective agentes
dc.titlePersistent oxytetracycline exposure induces an inflammatory process that improves regenerative capacity in zebrafish larvaees
dc.typeArtículoes
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