Transcription factor NAC1 activates expression of peptidase-encoding AtCEPs in roots to limit root hair growth

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dc.contributor.authorRodríguez-García, Diana R.
dc.contributor.authorRondón Guerrero, Yossmayer del Carmen
dc.contributor.authorFerrero, Lucía
dc.contributor.authorRossi, Andrés Hugo
dc.contributor.authorMiglietta, Esteban A.
dc.contributor.authorAptekmann, Ariel A.
dc.contributor.authorMarzol, Eliana
dc.contributor.authorPacheco, Javier Martínez
dc.contributor.authorCarignani, Mariana
dc.contributor.authorGabarain, Victoria Berdion
dc.contributor.authorLopez, Leonel E.
dc.contributor.authorDominguez, Gabriela Díaz
dc.contributor.authorBorassi, Cecilia
dc.contributor.authorSánchez-Serrano, José Juan
dc.contributor.authorXu, Lin
dc.contributor.authorNadra, Alejandro D.
dc.contributor.authorRojo, Enrique
dc.contributor.authorAriel, Federico
dc.contributor.authorEstevez, José M.
dc.date.accessioned2025-01-21T18:35:41Z
dc.date.available2025-01-21T18:35:41Z
dc.date.issued2024
dc.descriptionIndexación: Scopus
dc.description.abstractPlant genomes encode a unique group of papain-type Cysteine EndoPeptidases (CysEPs) containing a KDEL endoplasmic reticulum (ER) retention signal (KDEL-CysEPs or CEPs). CEPs process the cell-wall scaffolding EXTENSIN (EXT) proteins that regulate de novo cell-wall formation and cell expansion. Since CEPs cleave EXTs and EXT-related proteins, acting as cell-wall-weakening agents, they may play a role in cell elongation. The Arabidopsis (Arabidopsis thaliana) genome encodes 3 CEPs (AtCPE1-AtCEP3). Here, we report that the genes encoding these 3 Arabidopsis CEPs are highly expressed in root-hair (RH) cell files. Single mutants have no evident abnormal RH phenotype, but atcep1-3 atcep3-2 and atcep1-3 atcep2-2 double mutants have longer RHs than wild-type (Wt) plants, suggesting that expression of AtCEPs in root trichoblasts restrains polar elongation of the RH. We provide evidence that the transcription factor NAC1 (petunia NAM and Arabidopsis ATAF1, ATAF2, and CUC2) activates AtCEPs expression in roots to limit RH growth. Chromatin immunoprecipitation indicates that NAC1 binds to the promoter of AtCEP1, AtCEP2, and, to a lower extent, AtCEP3 and may directly regulate their expression. Inducible NAC1 overexpression increases AtCEP1 and AtCEP2 transcript levels in roots and leads to reduced RH growth while the loss of function nac1-2 mutation reduces AtCEP1-AtCEP3 gene expression and enhances RH growth. Likewise, expression of a dominant chimeric NAC1-SRDX repressor construct leads to increased RH length. Finally, we show that RH cell walls in the atcep1-3 atcep3-2 double mutant have reduced levels of EXT deposition, suggesting that the defects in RH elongation are linked to alterations in EXT processing and accumulation. Our results support the involvement of AtCEPs in controlling RH polar growth through EXT processing and insolubilization at the cell wall. © 2024 The Author(s).
dc.description.urihttps://academic-oup-com.recursosbiblioteca.unab.cl/plphys/article/194/1/81/7293258?login=true&token=eyJhbGciOiJub25lIn0.eyJleHAiOjE3NDAwNzUyNjYsImp0aSI6IjVlNjA2MjVkLTkzMjEtNGIwNC04OTY5LWE4ODZlOGQwYjIxZSJ9.
dc.identifier.citationPlant Physiology. Volume 194, Issue 1, Pages 81 - 93. January 2024
dc.identifier.doi10.1093/plphys/kiad533
dc.identifier.issn0032-0889
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/63138
dc.language.isoen
dc.publisherAmerican Society of Plant Biologists
dc.subjectArabidopsis
dc.subjectArabidopsis Proteins
dc.subjectGene Expression Regulation
dc.subjectPlant
dc.subjectPeptide Hydrolases
dc.subjectTranscription Factors
dc.subjectGenetics
dc.subjectMetabolism
dc.titleTranscription factor NAC1 activates expression of peptidase-encoding AtCEPs in roots to limit root hair growth
dc.typeArtículo
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