Deletion of VPS50 protein in mouse brain impairs synaptic function and behavior

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dc.contributor.authorAhumada-Marchant, Constanza
dc.contributor.authorAncatén-Gonzalez, Carlos
dc.contributor.authorHaensgen, Henny
dc.contributor.authorBrauer, Bastian
dc.contributor.authorMerino-Veliz, Nicolas
dc.contributor.authorDroste, Rita
dc.contributor.authorArancibia, Felipe
dc.contributor.authorHorvitz, H. Robert
dc.contributor.authorConstantine-Paton, Martha
dc.contributor.authorArriagada, Gloria
dc.contributor.authorChávez, Andrés E.
dc.contributor.authorBustos, Fernando J.
dc.date.accessioned2024-11-11T19:13:54Z
dc.date.available2024-11-11T19:13:54Z
dc.date.issued2024-12
dc.descriptionIndexación: Scopus.
dc.description.abstractBackground: The VPS50 protein functions in synaptic and dense core vesicle acidification, and perturbations of VPS50 function produce behavioral changes in Caenorhabditis elegans. Patients with mutations in VPS50 show severe developmental delay and intellectual disability, characteristics that have been associated with autism spectrum disorders (ASDs). The mechanisms that link VPS50 mutations to ASD are unknown. Results: To examine the role of VPS50 in mammalian brain function and behavior, we used the CRISPR/Cas9 system to generate knockouts of VPS50 in both cultured murine cortical neurons and living mice. In cultured neurons, KO of VPS50 did not affect the number of synaptic vesicles but did cause mislocalization of the V-ATPase V1 domain pump and impaired synaptic activity, likely as a consequence of defects in vesicle acidification and vesicle content. In mice, mosaic KO of VPS50 in the hippocampus altered synaptic transmission and plasticity and generated robust cognitive impairments. Conclusions: We propose that VPS50 functions as an accessory protein to aid the recruitment of the V-ATPase V1 domain to synaptic vesicles and in that way plays a crucial role in controlling synaptic vesicle acidification. Understanding the mechanisms controlling behaviors and synaptic function in ASD-associated mutations is pivotal for the development of targeted interventions, which may open new avenues for therapeutic strategies aimed at ASD and related conditions. © The Author(s) 2024.
dc.description.urihttps://bmcbiol.biomedcentral.com/articles/10.1186/s12915-024-01940-y
dc.identifier.doi10.1186/s12915-024-01940-y
dc.identifier.issn1741-7007
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/61871
dc.language.isoen
dc.publisherBMC Biology,Volume 22, Issue 1 December 2024, Article number 142
dc.rights.licenseAttribution 4.0 International CC BY 4.0 Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAnimals
dc.subjectBehavior, Animal
dc.subjectBrain
dc.subjectMice
dc.subjectMice, Knockout
dc.subjectNeurons
dc.subjectSynapses
dc.subjectSynaptic Transmission
dc.subjectSynaptic Vesicles
dc.subjectVacuolar Proton-Translocating ATPases
dc.subjectVesicular Transport Proteins
dc.titleDeletion of VPS50 protein in mouse brain impairs synaptic function and behavior
dc.typeArtículo
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