Piscirickettsia salmonis Produces a N-Acetyl-L-Homoserine Lactone as a Bacterial Quorum Sensing System-Related Molecule

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dc.contributor.authorRuiz, Pamela
dc.contributor.authorSepulveda, Daniela
dc.contributor.authorVidal, José Miguel
dc.contributor.authorRomero, Romina
dc.contributor.authorContreras, David
dc.contributor.authorBarros, Javier
dc.contributor.authorCarrasco, Carlos
dc.contributor.authorRuiz-Tagle, Nathaly
dc.contributor.authorRomero, Alex
dc.contributor.authorUrrutia, Homero
dc.contributor.authorOliver, Cristian
dc.date.accessioned2025-04-03T16:37:33Z
dc.date.available2025-04-03T16:37:33Z
dc.date.issued2021-10-25
dc.descriptionIndexación: Scopus.
dc.description.abstractPiscirickettsia salmonis is the etiological agent of piscirickettsiosis, the most prevalent disease in salmonid species in Chilean salmonids farms. Many bacteria produce N-acyl-homoserine lactones (AHLs) as a quorum-sensing signal molecule to regulate gene expression in a cell density-dependent manner, and thus modulate physiological characteristics and several bacterial mechanisms. In this study, a fluorescent biosensor system method and gas chromatography-tandem mass spectrometry (GC/MS) were combined to detect AHLs produced by P. salmonis. These analyses revealed an emitted fluorescence signal when the biosensor P. putida EL106 (RPL4cep) was co-cultured with both, P. salmonis LF-89 type strain and an EM-90-like strain Ps007, respectively. Furthermore, the production of an AHL-type molecule was confirmed by GC/MS by both P. salmonis strains, which identified the presence of a N-acetyl-L-homoserine Lactone in the supernatant extract. However, It is suggested that an alternate pathway could synthesizes AHLs, which should be address in future experiments in order to elucidate this important bacterial process. To the best of our knowledge, the present report is the first to describe the type of AHLs produced by P. salmonis. Copyright © 2021 Ruiz, Sepulveda, Vidal, Romero, Contreras, Barros, Carrasco, Ruiz-Tagle, Romero, Urrutia and Oliver.
dc.description.urihttps://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2021.755496/full
dc.identifier.citationFrontiers in Cellular and Infection Microbiology, Volume 11, 25 October 2021, Article number 755496
dc.identifier.doi10.3389/fcimb.2021.755496
dc.identifier.issn2235-2988
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/63965
dc.language.isoen
dc.publisherFrontiers Media S.A.
dc.rights.licenseAttribution 4.0 International CC BY 4.0 Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAHL (N-acyl-homoserine lactone)
dc.subjectN-acetyl-L-homoserine lactone
dc.subjectPiscirickettsia salmonis
dc.subjectQuorum sensing (QS)
dc.subjectSRS
dc.titlePiscirickettsia salmonis Produces a N-Acetyl-L-Homoserine Lactone as a Bacterial Quorum Sensing System-Related Molecule
dc.typeArtículo
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