HPV-18 E2 protein downregulates antisense noncoding mitochondrial RNA-2, delaying replicative senescence of human keratinocytes

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dc.contributor.authorVillota, Claudio
dc.contributor.authorVaras-Godoy, Manuel
dc.contributor.authorJeldes, Emanuel
dc.contributor.authorCampos, América
dc.contributor.authorVillegas, Jaime
dc.contributor.authorBorgna, Vincenzo
dc.contributor.authorBurzio, Luis O.
dc.contributor.authorBurzio, Verónica A.
dc.date.accessioned2023-10-10T14:34:31Z
dc.date.available2023-10-10T14:34:31Z
dc.date.issued2019-01-01
dc.descriptionIndexación: Scopuses
dc.description.abstractHuman and mouse cells display a differential expression pattern of a family of mitochondrial noncoding RNAs (ncmtRNAs), according to proliferative status. Normal proliferating and cancer cells express a sense ncmtRNA (SncmtRNA), which seems to be required for cell proliferation, and two antisense transcripts referred to as ASncmtRNA-1 and -2. Remarkably however, the ASncmtRNAs are downregulated in human and mouse cancer cells, including HeLa and SiHa cells, transformed with HPV-18 and HPV-16, respectively. HPV E2 protein is considered a tumor suppressor in the context of high-risk HPV-induced transformation and therefore, to explore the mechanisms involved in the downregulation of ASncmtRNAs during tumorigenesis, we studied human foreskin keratinocytes (HFK) transduced with lentiviral-encoded HPV-18 E2. Transduced cells displayed a significantly extended replicative lifespan of up to 23 population doublings, compared to 8 in control cells, together with downregulation of the ASncmtRNAs. At 26 population doublings, cells transduced with E2 were arrested at G2/M, together with downregulation of E2 and SncmtRNA and upregulation of ASncmtRNA-2. Our results suggest a role for high-risk HPV E2 protein in cellular immortalization. Additionally, we propose a new cellular phenotype according to the expression of the SncmtRNA and the ASncmtRNAs.es
dc.identifier.citationAging Volume 11, Issue 1, Pages 33 - 47 1 January 2019es
dc.identifier.doi10.18632/aging.101711en
dc.identifier.issn1945-4589
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/53424
dc.language.isoenes
dc.publisherAginges
dc.rights.licenseCC BY-ND 3.0 CL DEEDen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/cl/deed.esen
dc.subjectCervical canceres
dc.subjectG2 arrestes
dc.subjectHPV-18 E2es
dc.subjectMitochondrial ncRNAses
dc.subjectSenescencees
dc.titleHPV-18 E2 protein downregulates antisense noncoding mitochondrial RNA-2, delaying replicative senescence of human keratinocyteses
dc.typeArtículoes
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