Transport and secretion of the wnt3 ligand by motor neuron-like cells and developing motor neurons

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dc.contributor.authorPinto, Cristina
dc.contributor.authorPérez, Viviana
dc.contributor.authorMella, Jessica
dc.contributor.authorAlbistur, Miguel
dc.contributor.authorCaprile, Teresa
dc.contributor.authorBronfman, Francisca C.
dc.contributor.authorHenríquez, Juan Pablo
dc.date.accessioned2022-07-13T19:09:49Z
dc.date.available2022-07-13T19:09:49Z
dc.date.issued2021-12
dc.descriptionIndexación: Scopuses
dc.description.abstractThe vertebrate neuromuscular junction (NMJ) is formed by a presynaptic motor nerve terminal and a postsynaptic muscle specialization. Cumulative evidence reveals that Wnt ligands secreted by the nerve terminal control crucial steps of NMJ synaptogenesis. For instance, the Wnt3 ligand is expressed by motor neurons at the time of NMJ formation and induces postsynaptic differentiation in recently formed muscle fibers. However, the behavior of presynaptic-derived Wnt ligands at the vertebrate NMJ has not been deeply analyzed. Here, we conducted overexpression experiments to study the expression, distribution, secretion, and function of Wnt3 by transfection of the motor neuron-like NSC-34 cell line and by in ovo electroporation of chick motor neurons. Our findings reveal that Wnt3 is transported along motor axons in vivo following a vesicular-like pattern and reaches the NMJ area. In vitro, we found that endogenous Wnt3 expression increases as the differentiation of NSC-34 cells proceeds. Although NSC-34 cells overexpressing Wnt3 do not modify their morphological differentiation towards a neuronal phenotype, they effectively induce acetylcholine receptor clustering on co-cultured myotubes. These findings support the notion that presynaptic Wnt3 is transported and secreted by motor neurons to induce postsynaptic differentiation in nascent NMJs. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.es
dc.identifier.citationBiomolecules Volume 11, Issue 12December 2021 Article number 1898es
dc.identifier.doi10.3390/biom11121898
dc.identifier.issn2218273X
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/23228
dc.language.isoenes
dc.publisherBiomoleculeses
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.subjectMotor neurones
dc.subjectNeuromuscular junctiones
dc.subjectWntes
dc.titleTransport and secretion of the wnt3 ligand by motor neuron-like cells and developing motor neuronses
dc.typeArtículoes
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