Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions
| dc.contributor.author | Rasmussen-Poblete, Susana | |
| dc.contributor.author | Valdes, Jorge | |
| dc.contributor.author | Gamboa, Maria Cecilia | |
| dc.contributor.author | Valenzuela, Pablo D.T. | |
| dc.contributor.author | Krauskopf, Erwin | |
| dc.date.accessioned | 2014-03-21T16:32:20Z | |
| dc.date.accessioned | 2016-05-31T18:06:22Z | |
| dc.date.available | 2014-03-21T16:32:20Z | |
| dc.date.available | 2016-05-31T18:06:22Z | |
| dc.date.issued | 2008 | |
| dc.description | Indexación: Scielo | es |
| dc.description.abstract | Eucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs) are publicly available for this tree species. We constructed a cDNA from E. globulus seedlings subjected to low temperature and sequenced 9,913 randomly selected clones, generating 8,737 curated ESTs. The assembly produced 1,062 contigs and 3,879 singletons forming a Eucalyptus unigene set. Based on BLASTX analysis, 89.3% of the contigs and 88.5% of the singletons had significant similarity to known genes in the non-redundant database of GenBank. The Eucalyptus unigene set generated is a valuable public resource that provides an initial model for genes and regulatory pathways involved in cell wall biosynthesis at low temperature. | es |
| dc.description.sponsorship | Financial support: This work was partially funded by Universidad Andrés Bello (DI Proyect: 04-05/1) and MIFAB (Proyect: P04-071-F) and by the Microsoft Joint Research Program. | es |
| dc.identifier.citation | Electronic Journal of Biotechnology. Vol. 11. N° 2. Valparaíso, 2008. | es |
| dc.identifier.issn | 0717-3458 | |
| dc.identifier.uri | http://repositorio.unab.cl/xmlui/handle/ria/2424 | |
| dc.language.iso | en | es |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | es |
| dc.subject | cellulose, cold-temperature, EST database, forest biotechnology, lignin. | es |
| dc.title | Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions | es |
| dc.type | Artículo | es |
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