A role for Lin-28 in growth and metamorphosis in Drosophila melanogaster

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dc.contributor.authorGonzález-Itier, Sergio
dc.contributor.authorContreras, Esteban G.
dc.contributor.authorLarraín, Juan
dc.contributor.authorGlavic, Álvaro
dc.contributor.authorFaunes, Fernando
dc.date.accessioned2022-06-25T14:46:28Z
dc.date.available2022-06-25T14:46:28Z
dc.date.issued2018-12
dc.descriptionIndexación Scopuses
dc.description.abstractInsect metamorphosis has been a classic model to understand the role of hormones in growth and timing of developmental transitions. In addition to hormones, transitions in some species are regulated by genetic programs, such as the heterochronic gene network discovered in C. elegans. However, the functional link between hormones and heterochronic genes is not clear. The heterochronic gene lin-28 is involved in the maintenance of stem cells, growth and developmental timing in vertebrates. In this work, we used gain-of-function and loss-of-function experiments to study the role of Lin-28 in larval growth and the timing of metamorphosis of Drosophila melanogaster. During the late third instar stage, Lin-28 is mainly expressed in neurons of the central nervous system and in the intestine. Loss-of-function lin-28 mutant larvae are smaller and the larval-to-pupal transition is accelerated. This faster transition correlates with increased levels of ecdysone direct target genes such as Broad-Complex (BR-C) and Ecdysone Receptor (EcR). Overexpression of Lin-28 does not affect the timing of pupariation but most animals are not able to eclose, suggesting defects in metamorphosis. Overexpression of human Lin-28 results in delayed pupariation and the death of animals during metamorphosis. Altogether, these results suggest that Lin-28 is involved in the control of growth during larval development and in the timing and progression of metamorphosis. © 2018 Elsevier B.V.es
dc.description.urihttps://www-sciencedirect-com.recursosbiblioteca.unab.cl/science/article/pii/S0925477318300406?via%3Dihub
dc.identifier.citationMechanisms of Development Volume 154, Pages 107 - 115December 2018es
dc.identifier.doi10.1016/j.mod.2018.06.002
dc.identifier.issn09254773
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/22996
dc.language.isoenes
dc.publisherElsevier Ireland Ltdes
dc.subjectUTP-RNA Uridylyltransferasees
dc.subjectRNA-Binding Proteines
dc.subjectOligo(U)es
dc.titleA role for Lin-28 in growth and metamorphosis in Drosophila melanogasteres
dc.typeArtículoes
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