S. Typhimurium sseJ gene decreases the S. Typhi cytotoxicity toward cultured epithelial cells

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dc.contributor.authorTrombert, A Nicole
dc.contributor.authorBerrocal, Liliana
dc.contributor.authorFuentes, Juan A.
dc.contributor.authorMora, Guido C.
dc.date.accessioned2024-06-26T18:56:53Z
dc.date.available2024-06-26T18:56:53Z
dc.date.issued2010
dc.descriptionINDEXACIÓN: SCOPUS.
dc.description.abstractBackground. Salmonella enterica serovar Typhi and Typhimurium are closely related serovars as indicated by >96% DNA sequence identity between shared genes. Nevertheless, S. Typhi is a strictly human-specific pathogen causing a systemic disease, typhoid fever. In contrast, S. Typhimurium is a broad host range pathogen causing only a self-limited gastroenteritis in immunocompetent humans. We hypothesize that these differences have arisen because some genes are unique to each serovar either gained by horizontal gene transfer or by the loss of gene activity due to mutation, such as pseudogenes. S. Typhi has 5% of genes as pseudogenes, much more than S. Typhimurium which contains 1%. As a consequence, S. Typhi lacks several protein effectors implicated in invasion, proliferation and/or translocation by the type III secretion system that are fully functional proteins in S. Typhimurium. SseJ, one of these effectors, corresponds to an acyltransferase/lipase that participates in SCV biogenesis in human epithelial cell lines and is needed for full virulence of S. Typhimurium. In S. Typhi, sseJ is a pseudogene. Therefore, we suggest that sseJ inactivation in S. Typhi has an important role in the development of the systemic infection. Results. We investigated whether the S. Typhi trans-complemented with the functional sseJ gene from S. Typhimurium (STM) affects the cytotoxicity toward cultured cell lines. It was found that S. Typhi harbouring sseJ STMpresents a similar cytotoxicity level and intracellular retention/proliferation of cultured epithelial cells (HT-29 or HEp-2) as wild type S. Typhimurium. These phenotypes are significantly different from wild type S. Typhi. Conclusions. Based on our results we conclude that the mutation that inactivate the sseJ gene in S. Typhi resulted in evident changes in the behaviour of bacteria in contact with eukaryotic cells, plausibly contributing to the S. Typhi adaptation to the systemic infection in humans. © 2010 Trombert et al; licensee BioMed Central Ltd.
dc.identifier.citationBMC Microbiology, Volume 10, 2010, Article number 312
dc.identifier.doi10.1186/1471-2180-10-312
dc.identifier.issn1471-2180
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/57994
dc.language.isoen
dc.publisherBMC
dc.rights.licenseCC BY 2.0 ATTRIBUTION 2.0 GENERIC Deed
dc.rights.urihttps://creativecommons.org/licenses/by/2.0/
dc.subjectBacterial Proteins
dc.subjectCell Line
dc.subjectCell Proliferation
dc.subjectEpithelial Cells
dc.subjectGenetic Complementation Test
dc.subjectHumans
dc.subjectMutation
dc.subjectPseudogenes
dc.subjectSalmonella Infections
dc.subjectSalmonella typhi
dc.subjectSalmonella typhimurium
dc.subjectVirulence
dc.titleS. Typhimurium sseJ gene decreases the S. Typhi cytotoxicity toward cultured epithelial cells
dc.typeArtículo
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