Transcriptomic and Epigenomic Responses to Cortisol-Mediated Stress in Rainbow Trout (Oncorhynchus mykiss) Skeletal Muscle

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dc.contributor.authorAravena-Canales, Daniela
dc.contributor.authorValenzuela-Muñoz, Valentina
dc.contributor.authorGallardo-Escarate, Cristian
dc.contributor.authorMolina, Alfredo
dc.contributor.authorValdés, Juan Antonio
dc.date.accessioned2024-08-14T17:41:11Z
dc.date.available2024-08-14T17:41:11Z
dc.date.issued2024-07
dc.descriptionIndexación: Scopus.
dc.description.abstractThe production and release of cortisol during stress responses are key regulators of growth in teleosts. Understanding the molecular responses to cortisol is crucial for the sustainable farming of rainbow trout (Oncorhynchus mykiss) and other salmonid species. While several studies have explored the genomic and non-genomic impacts of cortisol on fish growth and skeletal muscle development, the long-term effects driven by epigenetic mechanisms, such as cortisol-induced DNA methylation, remain unexplored. In this study, we analyzed the transcriptome and genome-wide DNA methylation in the skeletal muscle of rainbow trout seven days after cortisol administration. We identified 550 differentially expressed genes (DEGs) by RNA-seq and 9059 differentially methylated genes (DMGs) via whole-genome bisulfite sequencing (WGBS) analysis. KEGG enrichment analysis showed that cortisol modulates the differential expression of genes associated with nucleotide metabolism, ECM-receptor interaction, and the regulation of actin cytoskeleton pathways. Similarly, cortisol induced the differential methylation of genes associated with focal adhesion, adrenergic signaling in cardiomyocytes, and Wnt signaling. Through integrative analyses, we determined that 126 genes showed a negative correlation between up-regulated expression and down-regulated methylation. KEGG enrichment analysis of these genes indicated participation in ECM-receptor interaction, regulation of actin cytoskeleton, and focal adhesion. Using RT-qPCR, we confirmed the differential expression of lamb3, itga6, limk2, itgb4, capn2, and thbs1. This study revealed for the first time the molecular responses of skeletal muscle to cortisol at the transcriptomic and whole-genome DNA methylation levels in rainbow trout.
dc.description.urihttps://www-scopus-com.recursosbiblioteca.unab.cl/record/display.uri?eid=2-s2.0-85199758967&origin=resultslist&sort=plf-f&src=s&sid=59bd6a7aecc65ab15ece3a097b4e218e&sot=aff&sdt=cl&cluster=scosubjabbr%2C%22COMP%22%2Ct%2Bscosubtype%2C%22ar%22%2Ct%2Bscoexactkeywords%2C%22Article%22%2Ct%2Bscofreetoread%2C%22all%22%2Ct&s=AF-ID%2860002636%29+AND+SUBJAREA%28COMP%29&sl=34&sessionSearchId=59bd6a7aecc65ab15ece3a097b4e218e&relpos=0
dc.identifier.citationInternational Journal of Molecular Sciences Open Access Volume 25, Issue 14 July 2024 Article number 7586
dc.identifier.doi10.3390/ijms25147586
dc.identifier.issn14220067
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/59257
dc.language.isoen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.rights.licenseCC BY 4.0 Attribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectcortisol
dc.subjectOncorhynchus mykiss
dc.subjectRNA-seq
dc.subjectskeletal muscle
dc.subjectWGBS
dc.titleTranscriptomic and Epigenomic Responses to Cortisol-Mediated Stress in Rainbow Trout (Oncorhynchus mykiss) Skeletal Muscle
dc.typeArtículo
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