Chalcone-induced apoptosis through caspase-dependent intrinsic pathways in human hepatocellular carcinoma cells

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dc.contributor.authorRamirez-Tagle, Rodrigo
dc.contributor.authorEscobar, Carlos A.
dc.contributor.authorRomero, Valentina
dc.contributor.authorMontorfano, Ignacio
dc.contributor.authorArmisén, Ricardo
dc.contributor.authorBorgna, Vincenzo
dc.contributor.authorJeldes, Emanuel
dc.contributor.authorPizarro, Luis
dc.contributor.authorSimon, Felipe
dc.contributor.authorEcheverria, Cesar
dc.date.accessioned2023-11-21T16:05:18Z
dc.date.available2023-11-21T16:05:18Z
dc.date.issued2016-02
dc.descriptionIndexación: Scopus.es
dc.description.abstractHepatocellular carcinoma (HCC) is one of the most commonly diagnosed cancers worldwide. Chemoprevention of HCC can be achieved through the use of natural or synthetic compounds that reverse, suppress or prevent the development of cancer progression. In this study, we investigated the antiproliferative effects and the mechanism of action of two compounds, 2,3,41 -trimethoxy-21 -hydroxy-chalcone (CH1) and 31 -bromo-3,4-dimethoxy-chalcone (CH2), over human hepatoma cells (HepG2 and Huh-7) and cultured mouse hepatocytes (HepM). Cytotoxic effects were observed over the HepG2 and Huh-7, and no effects were observed over the HepM. For HepG2 cells, treated separately with each chalcone, typical apoptotic laddering and nuclear condensation were observed. Additionally, the caspases and Bcl-2 family proteins activation by using Western blotting and immunocytochemistry were studied. Caspase-8 was not activated, but caspase-3 and -9 were both activated by chalcones in HepG2 cells. Chalcones also induced reactive oxygen species (ROS) accumulation after 4, 8 and 24 h of treatment in HepG2 cells. These results suggest that apoptosis in HepG2 was induced through: (i) a caspase-dependent intrinsic pathway; and (ii) by alterations in the cellular levels of Bcl-2 family proteins, and also, that the chalcone moiety could be a potent candidate as novel anticancer agents acting on human hepatomas.es
dc.description.urihttps://repositorio.unab.cl/xmlui/handle/ria/54007
dc.identifier.citationInternational Journal of Molecular Sciences. Volume 17, Issue. 222 February 2016. Article number 260es
dc.identifier.doi10.3390/ijms17020260
dc.identifier.issn1661-6596
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/54007
dc.language.isoenes
dc.publisherMDPI AGes
dc.rights.licenseAtribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectChalconees
dc.subjectCaspasees
dc.subjectReactive Oxygen Specieses
dc.titleChalcone-induced apoptosis through caspase-dependent intrinsic pathways in human hepatocellular carcinoma cellses
dc.typeArtículoes
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