Acidification triggers Andes hantavirus membrane fusion and rearrangement of Gc into a stable post-fusion homotrimer

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dc.contributor.authorAcuña, Rodrigo
dc.contributor.authorBignon, Eduardo A.
dc.contributor.authorMancini, Roberta
dc.contributor.authorLozach, Pierre-Yves
dc.contributor.authorTischler, Nicole D.
dc.date.accessioned2023-02-16T14:20:57Z
dc.date.available2023-02-16T14:20:57Z
dc.date.issued2015-11
dc.descriptionIndexación: Scopuses
dc.description.abstractThe hantavirus membrane fusion process is mediated by the Gc envelope glycoprotein from within endosomes. However, little is known about the specific mechanism that triggers Gc fusion activation, and its pre- and post-fusion conformations. We established cell-free in vitro systems to characterize hantavirus fusion activation. Low pH was sufficient to trigger the interaction of virus-like particles with liposomes. This interaction was dependent on a pre-fusion glycoprotein arrangement. Further, low pH induced Gc multimerization changes leading to non-reversible Gc homotrimers. These trimers were resistant to detergent, heat and protease digestion, suggesting characteristics of a stable post-fusion structure. No acid-dependent oligomerization rearrangement was detected for the trypsin-sensitive Gn envelope glycoprotein. Finally, acidification induced fusion of glycoprotein-expressing effector cells with non-susceptible CHO cells. Together, the data provide novel information on the Gc fusion trigger and its non-reversible activation involving lipid interaction, multimerization changes and membrane fusion which ultimately allow hantavirus entry into cells. © 2015 The Authors.es
dc.description.urihttps://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000269
dc.identifier.doi10.1099/jgv.0.000269
dc.identifier.issn0022-1317
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/46925
dc.language.isoenes
dc.publisherMicrobiology Societyes
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectHantaviruses
dc.subjectHemorrhagic Fever with Renal Syndromees
dc.subjectHantaviridae Infectionses
dc.titleAcidification triggers Andes hantavirus membrane fusion and rearrangement of Gc into a stable post-fusion homotrimeres
dc.typeArtículoes
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Journal of General Virology Volume 96, Issue 11, Pages 3192 - 3197November 2015
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