Wnt/β-catenin signaling stimulates the expression and synaptic clustering of the autism-associated Neuroligin 3 gene

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dc.contributor.authorMedina, M.A.
dc.contributor.authorAndrade, V.M.
dc.contributor.authorCaracci, M.O.
dc.contributor.authorAvila, M.E.
dc.contributor.authorVerdugo, D.A.
dc.contributor.authorVargas, M.F.
dc.contributor.authorUgarte, G.D.
dc.contributor.authorReyes, A.E.
dc.contributor.authorOpazo, C.
dc.contributor.authorDe Ferrari, G.V.
dc.date.accessioned2018-09-24T13:06:37Z
dc.date.available2018-09-24T13:06:37Z
dc.date.issued2018-12
dc.descriptionIndexación: Scopus.es_ES
dc.description.abstractSynaptic abnormalities have been described in individuals with autism spectrum disorders (ASD). The cell-adhesion molecule Neuroligin-3 (Nlgn3) has an essential role in the function and maturation of synapses and NLGN3 ASD-associated mutations disrupt hippocampal and cortical function. Here we show that Wnt/β-catenin signaling increases Nlgn3 mRNA and protein levels in HT22 mouse hippocampal cells and primary cultures of rat hippocampal neurons. We characterized the activity of mouse and rat Nlgn3 promoter constructs containing conserved putative T-cell factor/lymphoid enhancing factor (TCF/LEF)-binding elements (TBE) and found that their activity is significantly augmented in Wnt/β-catenin cell reporter assays. Chromatin immunoprecipitation (ChIP) assays and site-directed mutagenesis experiments revealed that endogenous β-catenin binds to novel TBE consensus sequences in the Nlgn3 promoter. Moreover, activation of the signaling cascade increased Nlgn3 clustering and co-localization with the scaffold PSD-95 protein in dendritic processes of primary neurons. Our results directly link Wnt/β-catenin signaling to the transcription of the Nlgn3 gene and support a functional role for the signaling pathway in the dysregulation of excitatory/inhibitory neuronal activity, as is observed in animal models of ASD.es_ES
dc.description.urihttps://www.nature.com/articles/s41398-018-0093-y.pdf
dc.identifier.citationTranslational Psychiatry, 8(1), art. no. 45.es_ES
dc.identifier.issn2158-3188
dc.identifier.otherDOI: 10.1038/s41398-018-0093-y
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/7032
dc.language.isoenes_ES
dc.publisherNature Publishing Groupes_ES
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBeta catenines_ES
dc.subjectDisks large homolog 4es_ES
dc.subjectMessenger RNAes_ES
dc.subjectT cell factor proteines_ES
dc.subjectWnt proteines_ES
dc.titleWnt/β-catenin signaling stimulates the expression and synaptic clustering of the autism-associated Neuroligin 3 genees_ES
dc.typeArtículoes_ES
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