Characterization and salt response in recurrent halotolerant exiguobacteriumsp. SH31 isolated from sediments of salar de huasco, chilean altiplano

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dc.contributor.authorRemonsellez, Francisco
dc.contributor.authorCastro-Severyn, Juan
dc.contributor.authorPardo-Esté, Coral
dc.contributor.authorAguilar, Pablo
dc.contributor.authorFortt, Jonathan
dc.contributor.authorSalinas, César
dc.contributor.authorBarahona, Sergio
dc.contributor.authorLeón, Joice
dc.contributor.authorFuentes, Bárbara
dc.contributor.authorAreche, Carlos
dc.contributor.authorHernández, Klaudia L.
dc.contributor.authorAguayo, Daniel
dc.contributor.authorSaavedra, Claudia P.
dc.date.accessioned2022-07-05T22:15:33Z
dc.date.available2022-07-05T22:15:33Z
dc.date.issued2018-09
dc.descriptionIndexación Scopuses
dc.description.abstractPoly-extremophiles microorganisms have the capacity to inhabit hostile environments and can survive several adverse conditions that include as variations in temperature, pH, and salinity, high levels UV light and atmospheric pressure, and even the presence of toxic compounds and the formation of reactive oxygen species (ROS). A halotolerant Exiguobacterium strain was isolated from Salar de Huasco (Chilean Altiplano), a well-known shallow lake area with variable salinity levels, little human intervention, and extreme environmental conditions, which makes it ideal for the study of resistant mechanisms and the evolution of adaptations. This bacterial genus has not been extensively studied, although its cosmopolitan location indicates that it has high levels of plasticity and adaptive capacity. However, to date, there are no studies regarding the tolerance and resistance to salinity and osmotic pressure. We set out to characterize the Exiguobacterium sp. SH31 strain and describe its phenotypical and genotypical response to osmotic stress. In this context, as a first step to characterize the response to the SH31 strain to salinity and to establish the bases for a molecular study, we proposed to compare its response under three salt conditions (0, 25, and 50 g/l NaCl). Using different physiology, genomic, and transcriptomic approaches, we determined that the bacterium is able to grow properly in a NaCl concentration of up to 50 g/l; however, the best growth rate was observed at 25 g/l. Although the presence of flagella is not affected by salinity, motility was diminished at 25 g/l NaCl and abolished at 50 g/l. Biofilm formation was induced proportionally with increases in salinity, which was expected. These phenotypic results correlated with the expression of related genes: fliG and fliS Motility); opuBA and putP (transport); glnA, proC, gltA, and gbsA (compatible solutes); ywqC, bdlA, luxS y pgaC (biofilm and stress response); and therefore, we conclude that this strain effectively modifies gene expression and physiology in a differential manner when faced with different concentrations of NaCl and these modifications aid survival. © 2007-2018 Frontiers Media S.A. All Rights Reserved.es
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2018.02228/full
dc.identifier.citationFrontiers in Microbiology Volume 9, Issue SEP20 September 2018 Article number 2228es
dc.identifier.doi10.3389/fmicb.2018.02228
dc.identifier.issn1664302X
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/23174
dc.language.isoenes
dc.publisherFrontiers Media S.A.es
dc.rights.licenseCC BY 4.0
dc.subjectExiguobacteriumes
dc.subjectDraftes
dc.subjectGenomees
dc.subjectChilean Altiplanoes
dc.subjectEnvironmental pressurees
dc.subjectExiguobacteriumes
dc.subjectExtremophilees
dc.titleCharacterization and salt response in recurrent halotolerant exiguobacteriumsp. SH31 isolated from sediments of salar de huasco, chilean altiplanoes
dc.typeArtículoes
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