Upon heat stress processing of ribosomal RNA precursors into mature rRNAs iscompromised after cleavage at primary P site in Arabidopsis thaliana

Vista sencilla de ítem
dc.contributor.authorDarriere T.
dc.contributor.authorJobet E.
dc.contributor.authorZavala D.
dc.contributor.authorEscande M.L.
dc.contributor.authorDurut N.
dc.contributor.authorde Bures A.
dc.contributor.authorBlanco-Herrera F.
dc.contributor.authorVidal E.A.
dc.contributor.authorRompais M.
dc.contributor.authorCarapito C.
dc.contributor.authorGourbiere S.
dc.contributor.authorSáez-Vásquez J.
dc.date.accessioned2024-08-16T17:17:20Z
dc.date.available2024-08-16T17:17:20Z
dc.date.issued2022
dc.descriptionIndexación: Scopus.
dc.description.abstractTranscription and processing of 45S rRNAs in the nucleolus are keystones of ribosome biogenesis. While these processes are severely impacted by stress conditions in multiple species, primarily upon heat exposure, we lack information about the molecular mechanisms allowing sessile organisms without a temperature-control system, like plants, to cope with such circumstances. We show that heat stress disturbs nucleolar structure, inhibits pre-rRNA processing and provokes imbalanced ribosome profiles in Arabidopsis thaliana plants. Notably, the accuracy of transcription initiation and cleavage at the primary P site in the 5’ETS (5’ External Transcribed Spacer) are not affected but the levels of primary 45S and 35S transcripts are, respectively, increased and reduced. In contrast, precursors of 18S, 5.8S and 25S RNAs are rapidly undetectable upon heat stress. Remarkably, nucleolar structure, pre-rRNAs from major ITS1 processing pathway and ribosome profiles are restored after returning to optimal conditions, shedding light on the extreme plasticity of nucleolar functions in plant cells. Further genetic and molecular analysis to identify molecular clues implicated in these nucleolar responses indicate that cleavage rate at P site and nucleolin protein expression can act as a checkpoint control towards a productive pre-rRNA processing pathway.
dc.description.urihttps://www.tandfonline.com/doi/epdf/10.1080/15476286.2022.2071517?src=getftr
dc.identifier.citationRNA Biology Volume 19, Issue 1, Pages 719 - 734 2022
dc.identifier.doi10.1080/15476286.2022.2071517
dc.identifier.issn1547-6286
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/59318
dc.language.isoen_US
dc.publisherTaylor and Francis Ltd.
dc.subjectArabidopsis
dc.subjectheat stress
dc.subjectNucleolus
dc.subjectribosome
dc.subjectrRNA processing
dc.titleUpon heat stress processing of ribosomal RNA precursors into mature rRNAs iscompromised after cleavage at primary P site in Arabidopsis thaliana
dc.typeArtículo
Archivos
Bloque original
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
Upon heat stress processing of ribosomal RNA precursors into mature rRNAs is compromised after cleavage at primary P site in Arabidopsis thaliana.pdf
Tamaño:
4.53 MB
Formato:
Adobe Portable Document Format
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista