The transcription factor SlyA from Salmonella Typhimurium regulates genes in response to hydrogen peroxide and sodium hypochlorite

Vista sencilla de ítem
dc.contributor.authorCabezas, Carolina E.
dc.contributor.authorBriones, Alan C.
dc.contributor.authorAguirre, Camila
dc.contributor.authorPardo-Esté, Coral
dc.contributor.authorCastro-Severyn, Juan
dc.contributor.authorSalinas, César R.
dc.contributor.authorBaquedano, María S.
dc.contributor.authorHidalgo, Alejandro A.
dc.contributor.authorFuentes, Juan A.
dc.contributor.authorMorales, Eduardo H.
dc.contributor.authorMeneses, Claudio A.
dc.contributor.authorCastro-Nallar, Eduardo
dc.contributor.authorSaavedra, Claudia Paz
dc.date.accessioned2022-07-28T22:43:54Z
dc.date.available2022-07-28T22:43:54Z
dc.date.issued2018-07
dc.descriptionIndexación Scopuses
dc.description.abstractSalmonella Typhimurium is an intracellular pathogen that is capable of generating systemic fever in a murine model. Over the course of the infection, Salmonella faces different kinds of stressors, including harmful reactive oxygen species (ROS). Various defence mechanisms enable Salmonella to successfully complete the infective process in the presence of such stressors. The transcriptional factor SlyA is involved in the oxidative stress response and invasion of murine macrophages. We evaluated the role of SlyA in response to H2O2 and NaOCl and found an increase of slyA expression upon exposure to these toxics. However, the SlyA target genes and the molecular mechanisms by which they influence the infective process are unknown. We hypothesised that SlyA regulates the expression of genes required for ROS resistance, metabolism, or virulence under oxidative stress conditions. Transcriptional profiling in wild type and ΔslyA strains confirmed that SlyA regulates the expression of several genes involved in virulence [sopD (STM14_3550), sopE2 (STM14_2244), hilA (STM14_3475)] and central metabolism [kgtP (STM14_3252), fruK (STM14_2722), glpA (STM14_2819)] in response to H2O2 and NaOCl. These findings were corroborated by functional assay and transcriptional fusion assays using GFP. DNA-protein interaction assays showed that SlyA regulates these genes through direct interaction with their promoter regions. © 2018 The Author(s)es
dc.description.urihttps://www-sciencedirect-com.recursosbiblioteca.unab.cl/science/article/pii/S0923250818300597?via%3Dihub
dc.identifier.citationResearch in Microbiology Volume 169, Issue 6, Pages 263 - 278July - August 2018es
dc.identifier.doi10.1016/j.resmic.2018.04.003
dc.identifier.issn09232508
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/23371
dc.language.isoenes
dc.publisherElsevier Masson SASes
dc.rights.licenseCC BY-NC-ND 4.0
dc.subjectSalmonella Enterica Serovar Typhimuriumes
dc.subjectVirulencees
dc.subjectBacterial Proteinses
dc.subjectDicarboxylic Acid Transporterses
dc.subjectGene Expression Profilinges
dc.titleThe transcription factor SlyA from Salmonella Typhimurium regulates genes in response to hydrogen peroxide and sodium hypochloritees
dc.typeArtículoes
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Cabezas_C_The_transcription_2018.pdf
Tamaño:
2.12 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLÉS
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista