Beyond purified dietary fibre supplements: Compositional variation between cell wall fibre from different plants influences human faecal microbiota activity and growth in vitro
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Fecha
2023-08
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
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Título del volumen
Editor
John Wiley and Sons Inc
Nombre de Curso
Licencia CC
Attribution 4.0 International Deed ( CC BY 4.0 ).
Licencia CC
https://creativecommons.org/licenses/by/4.0/
Resumen
Dietary fibre is a major energy source for the human gut microbiota, but it is unclear to what extent the fibre source and complexity affect microbial growth and metabolite production. Cell wall material and pectin were extracted from five different dicotyledon plant sources, apples, beet leaves, beetroots, carrots and kale, and compositional analysis revealed differences in the monosaccharide composition. Human faecal batch incubations were conducted with 14 different substrates, including the plant extracts, wheat bran and commercially available carbohydrates. Microbial activity was determined for up to 72 h by measuring gas and fermentation acid production, total bacteria (by qPCR) and microbial community composition by 16S rRNA amplicon sequencing. The more complex substrates gave rise to more microbiota variation compared with the pectins. The comparison of different plant organs showed that the leaves (beet leaf and kale) and roots (carrot and beetroot) did not give rise to similar bacterial communities. Rather, the compositional features of the plants, such as high arabinan levels in beet and high galactan levels in carrot, appear to be major predictors of bacterial enrichment on the substrates. Thus, in-depth knowledge on dietary fibre composition should aid the design of diets focused on optimizing the microbiota. © 2023 The Authors. Environmental Microbiology published by Applied Microbiology International and John Wiley & Sons Ltd.
Notas
Indexación: Scopus
Palabras clave
Bacteria, Dietary Fiber, Feces, Fermentation, Gastrointestinal Microbiome, Humans, Microbiota, Pectins, RNA, Ribosomal, 16S, Bacterium, Dietary Fiber, Feces, Fermentation, Genetics, Human, Intestine Flora, Metabolism, Microbiology, Microflora
Citación
Environmental Microbiology. Volume 25, Issue 8, Pages 1484 - 1504. August 2023
DOI
10.1111/1462-2920.16368