Persistent oxytetracycline exposure induces an inflammatory process that improves regenerative capacity in zebrafish larvae

Vista sencilla de ítem
dc.contributor.authorBarros-Becker, Francisco
dc.contributor.authorRomero, Jaime
dc.contributor.authorPulgar, Alvaro
dc.contributor.authorFeijóo, Carmen G.
dc.date.accessioned2024-04-09T21:08:08Z
dc.date.available2024-04-09T21:08:08Z
dc.date.issued2012-05-10
dc.descriptionINDEXACIÓN: SCOPUS.
dc.description.abstractBackground: The excessive use of antibiotics in aquaculture can adversely affect not only the environment, but also fish themselves. In this regard, there is evidence that some antibiotics can activate the immune system and reduce their effectiveness. None of those studies consider in detail the adverse inflammatory effect that the antibiotic remaining in the water may cause to the fish. In this work, we use the zebrafish to analyze quantitatively the effects of persistent exposure to oxytetracycline, the most common antibiotic used in fish farming. Methodology: We developed a quantitative assay in which we exposed zebrafish larvae to oxytetracycline for a period of 24 to 96 hrs. In order to determinate if the exposure causes any inflammation reaction, we evaluated neutrophils infiltration and quantified their total number analyzing the Tg(mpx:GFP)i114 transgenic line by fluorescence stereoscope, microscope and flow cytometry respectively. On the other hand, we characterized the process at a molecular level by analyzing several immune markers (il-1β, il-10, lysC, mpx, cyp1a) at different time points by qPCR. Finally, we evaluated the influence of the inflammation triggered by oxytetracycline on the regeneration capacity in the lateral line. Conclusions: Our results suggest that after 48 hours of exposure, the oxytetracycline triggered a widespread inflammation process that persisted until 96 hours of exposure. Interestingly, larvae that developed an inflammation process showed an improved regeneration capacity in the mechanosensory system lateral line. © 2012 Barros-Becker et al.
dc.identifier.citationPLoS ONE, Volume 7, Issue 5, 10 May 2012, Article number e36827
dc.identifier.doi10.1371/journal.pone.0036827
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/55795
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.licenseCC BY 4.0 DEED Atribución 4.0 Internacional
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectAnimals
dc.subjectAnimals, Genetically Modified
dc.subjectAnti-Bacterial Agents
dc.subjectBiological Markers
dc.subjectFish Diseases
dc.subjectInflammation
dc.subjectOxytetracycline
dc.subjectRegeneration
dc.subjectZebrafish
dc.titlePersistent oxytetracycline exposure induces an inflammatory process that improves regenerative capacity in zebrafish larvae
Archivos
Bloque original
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
Persistent-oxytetracycline-exposure-induces-an-inflammatory-process-that-improves-regenerative-capacity-in-zebrafish-larvaePLoS-ONE.pdf
Tamaño:
1.53 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLÉS.
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista