Subcellular location of Piscirickettsia salmonis heat shock protein 60 (Hsp60) chaperone by using immunogold labeling and proteomic analysis

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dc.contributor.authorOliver, C.
dc.contributor.authorSánchez, P.
dc.contributor.authorValenzuela, K.
dc.contributor.authorHernández, M.
dc.contributor.authorPontigo, J.
dc.contributor.authorRauch, M.
dc.contributor.authorGarduño, R.
dc.contributor.authorAvendaño-Herrera, R.
dc.contributor.authorYáñez, A.
dc.date.accessioned2021-09-29T16:12:03Z
dc.date.available2021-09-29T16:12:03Z
dc.date.issued2020
dc.descriptionIndexación: Scopus.es
dc.description.abstractPiscirickettsia salmonis is the causative bacterial agent of piscirickettsiosis, a systemic fish disease that significantly impacts the Chilean salmon industry. This bacterium possesses a type IV secretion system (T4SS), several proteins of the type III secretion system (T3SS), and a single heat shock protein 60 (Hsp60/GroEL). It has been suggested that due to its high antigenicity, the P. salmonis Hsp60 could be surface-exposed, translocated across the membrane, and (or) secreted into the extracellular matrix. This study tests the hypothesis that P. salmonis Hsp60 could be located on the bacterial surface. Immunogold electron microscopy and proteomic analyses suggested that although P. salmonis Hsp60 was predominantly associated with the bacterial cell cytoplasm, Hsp60-positive spots also exist on the bacterial cell envelope. IgY antibodies against P. salmonis Hsp60 protected SHK-1 cells against infection. Several bioinformatics approaches were used to assess Hsp60 translocation by the T4SS, T3SS, and T6SS, with negative results. These data support the hypothesis that small amounts of Hsp60 must reach the bacterial cell surface in a manner probably not mediated by currently characterized secretion systems, and that they remain biologically active during P. salmonis infection, possibly mediating adherence and (or) invasion.es
dc.description.urihttps://www.mdpi.com/2076-2607/8/1/117
dc.identifier.citationMicroorganisms, Volume 8, Issue 1, January 2020, Article number 117es
dc.identifier.doiDOI: 10.3390/microorganisms8010117
dc.identifier.issn2076-2607
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/20382
dc.language.isoenes
dc.publisherMDPI AGes
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.subjectFish pathologyes
dc.subjectIgYes
dc.subjectPiscirickettsia salmonises
dc.subjectSecretion systemses
dc.subjectSRSes
dc.subjectVaccinationes
dc.subjectPiscirickettsia Salmonises
dc.subjectFrancisellaes
dc.subjectRickettsia-like Organismses
dc.titleSubcellular location of Piscirickettsia salmonis heat shock protein 60 (Hsp60) chaperone by using immunogold labeling and proteomic analysises
dc.typeArtículoes
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