Azorella compacta infusion activates human immune cells and scavenges free radicals in vitro

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dc.contributor.authorTumová, L.
dc.contributor.authorDučaiová, Z.
dc.contributor.authorCheel, J.
dc.contributor.authorVokřál, I.
dc.contributor.authorSepúlveda, B.
dc.contributor.authorVokurková, D.
dc.date.accessioned2017-10-26T16:41:56Z
dc.date.available2017-10-26T16:41:56Z
dc.date.issued2017
dc.descriptionIndexación: Scopus.es_CL
dc.description.abstractBackground: Azorella compacta is traditionally used in the form of tea (infusion), in the Andean region of South America, to treat various chronic diseases. However, the health-promoting properties of this herbal tea have not yet been extensively explored. Materials and Methods: The free radical scavenging activity of A. compacta infusion (ACI) was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and superoxide anion radical assays. The activation of immune cells by ACI, as determined by cell surface cluster of differentiation 69 expression, was measured by flow cytometry. The qualitative polyphenolic composition of ACI was investigated by HPLC/PDA/ESI-MS, (High-performance liquid chromatography coupled with photodiode array detection and electrospray ionization-mass spectrometry) and the total content of polyphenols was estimated by spectrophotometric methods. Results: Eight polyphenols including chlorogenic acid, 6,8-di-C-hexosyl apigenin, isoorientin, orientin, dicaffeoylquinic acid, biochanin A-O-glucoside, biochanin A-O-(malonyl)-glucoside, and licoisoflavone A were tentatively identified in ACI. The total contents of phenols, flavonoids, and tannins in lyophilized ACI were 5.40 mg/100 mg ACI, 1.79 mg/100 mg ACI, and 1.76 mg/100 mg ACI, respectively. ACI, within the range of 25-400 μg/mL, scavenged DPPH and O2 .- by 15-90% and 20-88%, respectively. The human natural killer (NK) cells were substantially activated by ACI, whereas T cells and granulocytes were slightly stimulated. Conclusion: Overall, the results demonstrate the free radical scavenging and immune-stimulating properties of ACI, and support, at least in part, its potential utilization as a functional herbal tea. for preventing chronic diseases and as a nonspecific immune stimulator during human immunosenescence.es_CL
dc.description.urihttp://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=50;spage=260;epage=264;aulast=Tumov%E1
dc.identifier.citationPharmacognosy Magazine. Volume 13, Issue 50, April-June 2017, Pages 260-264es_CL
dc.identifier.issn0973-1296
dc.identifier.otherDOI: 10.4103/0973-1296.204558
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/4479
dc.language.isoenes_CL
dc.publisherMedknow Publicationses_CL
dc.subjectAzorella compactaes_CL
dc.subjectCD69 expressiones_CL
dc.subjectFree radicals scavenging activityes_CL
dc.subjectImmune cellses_CL
dc.subjectPhenolices_CL
dc.titleAzorella compacta infusion activates human immune cells and scavenges free radicals in vitroes_CL
dc.typeArtículoes_CL
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