Estrogen and progesterone up-regulate glucose transporter expression in ZR-75-1 human breast cancer cells

Vista sencilla de ítem
dc.contributor.authorMedina, R.
dc.contributor.authorMeneses, A.
dc.contributor.authorVera, J.
dc.contributor.authorGuzman, C.
dc.contributor.authorNualart, F.
dc.contributor.authorAstuya, A.
dc.contributor.authorGarcía, M.
dc.contributor.authorKato, S.
dc.contributor.authorCarvajal, A.
dc.contributor.authorPinto, M.
dc.contributor.authorOwen, G.
dc.date.accessioned2021-06-02T04:25:49Z
dc.date.available2021-06-02T04:25:49Z
dc.date.issued2003-10
dc.descriptionIndexación: Scopus.es
dc.description.abstractBreast cancer incidence increases in women receiving combined estrogen and progesterone therapy. Breast tumors show increased expression of the glucose transporter GLUT1. We determined the effect of these hormones on GLUT1-4 expression and deoxyglucose transport in ZR-75-1 breast cancer cells. Immunoblotting, immunocytochemistry, flow cytometry, and RT-PCR showed that GLUT1 expression is up-regulated by progesterone and, to a greater degree, combined therapy. GLUT2 expression is unaffected by hormonal treatment. GLUT3 protein and RNA is up-regulated by progesterone and combined therapy, and GLUT4 protein expression is up-regulated by all hormonal treatments. Deoxyglucose transport studies revealed the presence of three transport components with characteristics corresponding to GLUT1/4, GLUT2, and GLUT3. 17β-Estradiol produced a slight increase in transport at the Michaelis constant (Km) corresponding to GLUT3. Progesterone produced a small increase in transport at the Km corresponding to GLUT1/4, and combined 17β-estradiol and progesterone produced a small increase in transport at the Km corresponding to GLUT3 and a large increase in transport at the Km corresponding to GLUT1/4. This indicates that 17β-estradiol and progesterone differentially regulate GLUT1-4 expression and that these changes correlate to changes in glucose uptake. We postulate that combined hormone replacement therapy provides a survival advantage to developing ZR-75 breast cancer cells.es
dc.description.urihttps://academic.oup.com/endo/article/144/10/4527/2502364
dc.identifier.citationEndocrinology ,Volume 144, Issue 10, 1 October 2003, Pages 4527-4535es
dc.identifier.doiDOI: 10.1210/en.2003-0294
dc.identifier.issn0013-7227
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/18996
dc.language.isoenes
dc.publisherOxford University Presses
dc.subjectMaximum Standardized Uptake Valuees
dc.subjectPhloretines
dc.subjectGlucose Transporterses
dc.subjectdeoxyglucosees
dc.subjectestradioles
dc.subjectestrogenes
dc.subjectbreast carcinomaes
dc.subjectBreast Neoplasmses
dc.titleEstrogen and progesterone up-regulate glucose transporter expression in ZR-75-1 human breast cancer cellses
dc.typeArtículoes
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Medina_ Estrogen and Progesterone Up-Regulate Glucose Transporter Expression in ZR.pdf
Tamaño:
560.99 KB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLES
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista