Florfenicol Binding to Molecularly Imprinted Polymer Nanoparticles in Model and Real Samples

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dc.contributor.authorCaro, Nelson
dc.contributor.authorBruna, Tamara
dc.contributor.authorGuerreiro, Antonio
dc.contributor.authorAlvarez-Tejos, Paola
dc.contributor.authorGarretón, Virginia
dc.contributor.authorPiletsky, Sergey
dc.contributor.authorGonzález-Casanova, Jorge
dc.contributor.authorRojas-Gómez, Diana
dc.contributor.authorEhrenfeld, Nicole
dc.date.accessioned2021-10-27T13:41:50Z
dc.date.available2021-10-27T13:41:50Z
dc.date.issued2020-02
dc.description.abstractA simple and straightforward technique for coating microplate wells with molecularly imprinted polymer nanoparticles (nanoMIPs) to develop assays similar to the enzyme-linked immunosorbent (ELISA) assay to determine and quantify florfenicol (FF) in real food samples such as liquid milk and salmon muscle is presented here. The nanoMIPs were synthesized by a solid-phase approach with an immobilized FF (template) and characterized using dynamic light scattering, a SPR-2 biosensor system and transmission electron microscopy. Immobilization of nanoMIPs was conducted by preparing a homogenous solution of FF-nanoMIPs in water mixed with polyvinyl alcohol (PVA) 0.2% (w/v) in each well of a microplate. The detection of florfenicol was achieved in competitive binding experiments with a horseradish peroxidase−florfenicol (FF-HRP) conjugate. The assay made it possible to measure FF in buffer and in real samples (liquid milk and salmon muscle) within the range of 60−80 and 90–100 ng/mL, respectively. The immobilized nanoMIPs were stored for six weeks at room temperature and at 5 °C. The results indicate good signal recovery for all FF concentrations in spiked milk samples, without any detrimental effects to their binding properties. The high affinity of nanoMIPs and the lack of a requirement for cold chain logistics make them an attractive alternative to traditional antibodies used in ELISA. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.es
dc.description.sponsorshipIndexación: Scopuses
dc.identifier.citationNanomaterials Open Access Volume 10, Issue 2February 2020 Article number 306es
dc.identifier.issn20794991
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/20658
dc.language.isoenes
dc.publisherMDPI AGes
dc.subjectFlorfenicol; Nanoparticles; Polymeres
dc.titleFlorfenicol Binding to Molecularly Imprinted Polymer Nanoparticles in Model and Real Sampleses
dc.typeArtículoes
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