Unveiling Novel Kunitz- and Waprin-Type Toxins in the Micrurus mipartitus Coral Snake Venom Gland: An In Silico Transcriptome Analysis

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dc.contributor.authorSaldarriaga-Córdoba, Mónica
dc.contributor.authorClavero-León, Claudia
dc.contributor.authorRey-Suarez, Paola
dc.contributor.authorNuñez-Rangel, Vitelbina
dc.contributor.authorAvendaño-Herrera, Ruben
dc.contributor.authorSolano-González, Stefany
dc.contributor.authorAlzate, Juan F.
dc.date.accessioned2024-07-09T20:36:29Z
dc.date.available2024-07-09T20:36:29Z
dc.date.issued2024-06
dc.descriptionIndexación: Scopus.
dc.description.abstractKunitz-type peptide expression has been described in the venom of snakes of the Viperidae, Elapidae and Colubridae families. This work aimed to identify these peptides in the venom gland transcriptome of the coral snake Micrurus mipartitus. Transcriptomic analysis revealed a high diversity of venom-associated Kunitz serine protease inhibitor proteins (KSPIs). A total of eight copies of KSPIs were predicted and grouped into four distinctive types, including short KSPI, long KSPI, Kunitz–Waprin (Ku-WAP) proteins, and a multi-domain Kunitz-type protein. From these, one short KSPI showed high identity with Micrurus tener and Austrelaps superbus. The long KSPI group exhibited similarity within the Micrurus genus and showed homology with various elapid snakes and even with the colubrid Pantherophis guttatus. A third group suggested the presence of Kunitz domains in addition to a whey-acidic-protein-type four-disulfide core domain. Finally, the fourth group corresponded to a transcript copy with a putative 511 amino acid protein, formerly annotated as KSPI, which UniProt classified as SPINT1. In conclusion, this study showed the diversity of Kunitz-type proteins expressed in the venom gland transcriptome of M. mipartitus.
dc.description.urihttps://www-scopus-com.recursosbiblioteca.unab.cl/record/display.uri?eid=2-s2.0-85194125706&origin=resultslist&sort=plf-f&src=s&nlo=&nlr=&nls=&sid=a3f5670d50081e2718623b65a230bcce&sot=aff&sdt=cl&cluster=scofreetoread%2c%22all%22%2ct&sl=34&s=AF-ID%2860002636%29+AND+SUBJAREA%28ENVI%29&relpos=6&citeCnt=0&searchTerm=
dc.identifier.citationToxins Open Access Volume 16, Issue 5 May 2024 Article number 224
dc.identifier.doi10.3390/toxins16050224
dc.identifier.issn20726651
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/58392
dc.language.isoen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.rights.licenseCC BY 4.0 ATTRIBUTION 4.0 INTERNATIONAL
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectKSPI
dc.subjectmanual curations
dc.subjectMicrurus mipartitus
dc.subjectnovel Kunitz-type inhibitor
dc.subjectvenom gland transcriptome
dc.titleUnveiling Novel Kunitz- and Waprin-Type Toxins in the Micrurus mipartitus Coral Snake Venom Gland: An In Silico Transcriptome Analysis
dc.typeArtículo
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Saldarriaga-C-Unveiling-Novel-Kunitz-and-WaprinType-Toxins-in-the-Micrurusmipartitus-Coral-Snake-Venom-Gland-An-In-Silico-Transcriptome-Analysis.pdf
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