Loss of caveolin-1 is associated with a decrease in beta cell death in mice on a high fat diet

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dc.contributor.authorUrzúa, P.L.
dc.contributor.authorMurillo, O.N.
dc.contributor.authorCastro-Sepúlveda, M.
dc.contributor.authorTorres-Quintana, M.A.
dc.contributor.authorCaldera, Á.L.
dc.contributor.authorQuest, A.F.G.
dc.contributor.authorRobles, C.E.
dc.contributor.authorVidal, P.L.
dc.contributor.authorWehinger, S.
dc.date.accessioned2021-09-04T15:40:49Z
dc.date.available2021-09-04T15:40:49Z
dc.date.issued2020-08
dc.descriptionIndexación: Scopus.es
dc.description.abstractElevated free fatty acids (FFAs) impair beta cell function and reduce beta cell mass as a consequence of the lipotoxicity that occurs in type 2 diabetes (T2D). We previously reported that the membrane protein caveolin-1 (CAV1) sensitizes to palmitate-induced apoptosis in the beta pancreatic cell line MIN6. Thus, our hypothesis was that CAV1 knock-out (CAV1 KO) mice subjected to a high fat diet (HFD) should suffer less damage to beta cells than wild type (WT) mice. Here, we evaluated the in vivo response of beta cells in the pancreatic islets of 8-week-old C57Bl/6J CAV1 KO mice subjected to a control diet (CD, 14% kcal fat) or a HFD (60% kcal fat) for 12 weeks. We observed that CAV1 KO mice were resistant to weight gain when on HFD, although they had high serum cholesterol and FFA levels, impaired glucose tolerance and were insulin resistant. Some of these alterations were also observed in mice on CD. Interestingly, KO mice fed with HFD showed an adaptive response of the pancreatic beta cells and exhibited a significant decrease in beta cell apoptosis in their islets compared to WT mice. These in vivo results suggest that although the CAV1 KO mice are metabolically unhealthy, they adapt better to a HFD than WT mice. To shed light on the possible signaling pathway(s) involved, MIN6 murine beta cells expressing (MIN6 CAV) or not expressing (MIN6 Mock) CAV1 were incubated with the saturated fatty acid palmitate in the presence of mitogen-activated protein kinase inhibitors. Western blot analysis revealed that CAV1 enhanced palmitate-induced JNK, p38 and ERK phosphorylation in MIN6 CAV1 cells. Moreover, all the MAPK inhibitors partially restored MIN6 viability, but the effect was most notable with the ERK inhibitor. In conclusion, our results suggest that CAV1 KO mice adapted better to a HFD despite their altered metabolic state and that this may at least in part be due to reduced beta cell damage. Moreover, they indicate that the ability of CAV1 to increase sensitivity to FFAs may be mediated by MAPK and particularly ERK activation. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.es
dc.description.urihttps://www.mdpi.com/1422-0067/21/15/5225
dc.identifier.citationInternational Journal of Molecular SciencesOpen AccessVolume 21, Issue 15, Pages 1 - 191 August 2020 Article number 5225es
dc.identifier.doi10.3390/ijms21155225
dc.identifier.issn1661-6596
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/20116
dc.language.isoenes
dc.publisherMDPI AGes
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCaveolin-1es
dc.subjectDiabeteses
dc.subjectERK activityes
dc.subjectHigh fat dietes
dc.subjectInsulin resistancees
dc.titleLoss of caveolin-1 is associated with a decrease in beta cell death in mice on a high fat dietes
dc.typeArtículoes
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