Microbial communities associated with farmed Genypterus chilensis: Detection in water prior to bacterial outbreaks using culturing high-throughput sequencing

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dc.contributor.authorLevican, Arturo
dc.contributor.authorFisher, Jenny C.
dc.contributor.authorMcLellan, Sandra L.
dc.contributor.authorAvendaño-Herrera, Ruben
dc.date.accessioned2023-11-11T14:22:13Z
dc.date.available2023-11-11T14:22:13Z
dc.date.issued2020-06
dc.descriptionIndexación: Scopuses
dc.description.abstractThe red conger eel (Genypterus chilensis, Guichenot) is a native species included in the Chilean Aquaculture Diversification Program due to high commercial demand. In the context of intensified farming, prior reports link two disease outbreaks with emerging pathogens in the Vibrio and Tenacibaculum genera. However, the roles remain unclear for the bacterial community and each specific bacterium is associated with the rearing environment for healthy specimens. The success of red conger eel farming therefore warrants research into the bacterial composition of aquaculture conditions and the antimicrobial susceptibilities thereof. This study used culturing methods and high-throughput sequencing to describe the bacterial community associated with water in which G. chilensis was farmed. With culturing methods, the predominant genera were Vibrio (21.6%), Pseudolteromonas (15.7%), Aliivibrio (13.7%), and Shewanella (7.8%). Only a few bacterial isolates showed amylase, gelatinase, or lipase activity, and almost all showed inhibition zones to commonly- used antibiotics in aquaculture. By contrast, high-throughput sequencing established Paraperlucidibaca, Colwellia, Polaribacter, Saprospiraceae, and Tenacibaculum as the predominant genera, with Vibrio ranking twenty-seventh in abundance. High-throughput sequencing also established a link between previous outbreaks with increased relative abundances of Vibrio and Tenacibaculum. Therefore, monitoring the presence and abundance of these potential pathogens could be useful in providing prophylactic measures to prevent future outbreaks.es
dc.identifier.citationAnimals Volume 10, Issue 6, Pages 1 - 17 June 2020 Article number 1055es
dc.identifier.doi10.3390/ani10061055en
dc.identifier.issn2076-2615
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/53926
dc.language.isoenes
dc.publisherMDPI AGes
dc.rights.licenseCC BY 4.0 DEED Atribución 4.0 Internacionalen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.esen
dc.subjectGenypterus chilensises
dc.subjectHigh throughput sequencinges
dc.subjectPlate countinges
dc.subjectTenacibaculum sppes
dc.subjectVibrio sppes
dc.titleMicrobial communities associated with farmed Genypterus chilensis: Detection in water prior to bacterial outbreaks using culturing high-throughput sequencinges
dc.typeArtículoes
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