SPI-9 of Salmonella enterica serovar typhi is constituted by an operon positively regulated by RpoS and contributes to adherence to epithelial cells in culture

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dc.contributor.authorVelásquez, Juan C.
dc.contributor.authorHidalgo, Alejandro A.
dc.contributor.authorVillagra, Nicolás
dc.contributor.authorSantiviago, Carlos A.
dc.contributor.authorMora, Guido C.
dc.contributor.authorFuentes, Juan A.
dc.date.accessioned2023-10-02T18:32:05Z
dc.date.available2023-10-02T18:32:05Z
dc.date.issued2016-08
dc.descriptionIndexación: Scopus.es
dc.description.abstractThe genomic island 9 (SPI-9) from Salmonella enterica serovar Typhi (S. Typhi) carries three ORFs (STY2876, STY2877, STY2878) presenting 98 % identity with a type 1 secretory apparatus (T1SS), and a single ORF (STY2875) similar to a large RTX-like protein exhibiting repeated Ig domains. BapA, the Salmonella enterica serovar Enteritidis orthologous to S. Typhi STY2875, has been associated with biofilm formation, and is described as a virulence factor in mice. Preliminary in silico analyses revealed that S. Typhi STY2875 ORF has a 600 bp deletion compared with S. Enteritidis bapA, suggesting that S. Typhi STY2875 might be non-functional. At present, SPI-9 has not been studied in S. Typhi. We found that the genes constituting SPI-9 are arranged in an operon whose promoter was up-regulated in high osmolarity and low pH in a RpoS-dependent manner. All the proteins encoded by S. Typhi SPI-9 were located at the membrane fraction, consistent with their putative role as T1SS. Furthermore, SPI-9 contributed to adherence of S. Typhi to epithelial cells when bacteria were grown under high osmolarity or low pH. Under the test conditions, S. Typhi SPI-9 did not participate in biofilm formation. SPI-9 is functional in S. Typhi and encodes an adhesin induced under conditions normally found in the intestine, such as high osmolarity. Hence, this is an example of a locus that might be designated a pseudogene by computational approaches but not by direct biological assays.es
dc.description.urihttps://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000319
dc.identifier.citationMicrobiology (United Kingdom). Volume 162, Issue 8, Pages 1367 - 1378. August 2016es
dc.identifier.doi10.1099/mic.0.000319
dc.identifier.issn1350-0872
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/53393
dc.language.isoenes
dc.publisherMicrobiology Societyes
dc.subjectSalmonellaTyphies
dc.subjectSistema de Secreción Tipo unoes
dc.subjectAdherenciaes
dc.subjectAdhesinaes
dc.titleSPI-9 of Salmonella enterica serovar typhi is constituted by an operon positively regulated by RpoS and contributes to adherence to epithelial cells in culturees
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