Using a ligate intestinal loop mouse model to investigate Clostridioides difficile adherence to the intestinal mucosa in aged mice
Loading...
Date
2021-12
Profesor/a GuÃa
Facultad/escuela
Idioma
en
Journal Title
Journal ISSN
Volume Title
Publisher
Public Library of Science
Nombre de Curso
item.page.dc.rights
Atribution 4.0 International (CC BY 4.0)
item.page.dc.rights
https://creativecommons.org/licenses/by/4.0/deed.es
Abstract
Interaction of Clostridioides difficile spores with the intestinal mucosa contributes to the persistence and recurrence of the infection. Advanced age is one of the main risk factors for C. difficile infection and recurrence of the disease. However, interaction of C. difficile spores with the intestinal mucosa during aging has not been evaluated. In the present work, using intestinal ligated loop technique in a mouse model, we analyzed C. difficile spore adherence and internalization to the ileum and colonic mucosa during aging. Additionally, we provide visual documentation of the critical steps of the procedure. Consequently, our data suggest that spore internalization in the ileum and colonic mucosa is higher in elderly mice rather than adults or young mice. Also, our data suggest that spore adherence to the ileum and colonic mucosa decreases with aging.
item.page.dc.description
Indexación: Scopus.
Keywords
Gastrointestinal Tract, Bacterial Spores, Colon, Ileum, Aging, Surgical and Invasive Medical Procedures, Confocal Microscopy, Mouse Models
Citation
PLoS ONE. Volume 16, Issue 12 December. December 2021. Article number e0261081
DOI
10.1371/journal.pone.0261081