Genome analysis of the biotechnologically relevant acidophilic iron oxidising strain JA12 indicates phylogenetic and metabolic diversity within the novel genus "Ferrovum"

Vista sencilla de ítem
dc.contributor.authorUllrich, Sophie R.
dc.contributor.authorPoehlein, Anja
dc.contributor.authorTischler, Judith S.
dc.contributor.authorGonzález, Carolina
dc.contributor.authorOssandon, Francisco J.
dc.contributor.authorDaniel, Rolf
dc.contributor.authorHolmes, David S.
dc.contributor.authorSchlömann, Michael
dc.contributor.authorMühling, Martin
dc.date.accessioned2024-04-10T17:36:33Z
dc.date.available2024-04-10T17:36:33Z
dc.date.issued2016-01
dc.descriptionINDEXACIÓN: SCOPUS.
dc.description.abstractBackground: Members of the genus "Ferrovum" are ubiquitously distributed in acid mine drainage (AMD) waters which are characterised by their high metal and sulfate loads. So far isolation and microbiological characterisation have only been successful for the designated type strain "Ferrovum myxofaciens" P3G. Thus, knowledge about physiological characteristics and the phylogeny of the genus "Ferrovum" is extremely scarce. Objective: In order to access the wider genetic pool of the genus "Ferrovum" we sequenced the genome of a "Ferrovum"-containing mixed culture and successfully assembled the almost complete genome sequence of the novel "Ferrovum" strain JA12. Phylogeny and lifestyle: The genome-based phylogenetic analysis indicates that strain JA12 and the type strain represent two distinct "Ferrovum" species. "Ferrovum" strain JA12 is characterised by an unusually small genome in comparison to the type strain and other iron oxidising bacteria. The prediction of nutrient assimilation pathways suggests that "Ferrovum" strain JA12 maintains a chemolithoautotrophic lifestyle utilising carbon dioxide and bicarbonate, ammonium and urea, sulfate, phosphate and ferrous iron as carbon, nitrogen, sulfur, phosphorous and energy sources, respectively. Unique Metabolic Features: The potential utilisation of urea by "Ferrovum" strain JA12 is moreover remarkable since it may furthermore represent a strategy among extreme acidophiles to cope with the acidic environment. Unlike other acidophilic chemolithoautotrophs "Ferrovum" strain JA12 exhibits a complete tricarboxylic acid cycle, a metabolic feature shared with the closer related neutrophilic iron oxidisers among the Betaproteobacteria including Sideroxydans lithotrophicus and Thiobacillus denitrificans. Furthermore, the absence of characteristic redox proteins involved in iron oxidation in the well-studied acidophiles Acidithiobacillus ferrooxidans (rusticyanin) and Acidithiobacillus ferrivorans (iron oxidase) indicates the existence of a modified pathway in "Ferrovum" strain JA12. Therefore, the results of the present study extend our understanding of the genus "Ferrovum" and provide a comprehensive framework for future comparative genome and metagenome studies. © 2016 Ullrich et al.
dc.identifier.citationPLoS ONE, Volume 11, Issue 1, January 2016, Article number e0146832
dc.identifier.doi10.1371/journal.pone.0146832
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/55853
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.licenseCC BY 4.0 DEED Atribución 4.0 Internacional
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectBetaproteobacteria
dc.subjectDNA, Bacterial
dc.subjectGenome, Bacterial
dc.subjectIron
dc.subjectMetagenome
dc.subjectMining
dc.subjectPhylogeny
dc.titleGenome analysis of the biotechnologically relevant acidophilic iron oxidising strain JA12 indicates phylogenetic and metabolic diversity within the novel genus "Ferrovum"
Archivos
Bloque original
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
Genome-analysis-of-the-biotechnologically-relevant-acidophilic-iron-oxidising-strain-JA12-indicates-phylogenetic-and-metabolic-diversity-within-the-novel-genus-FerrovumPLoS-ONE.pdf
Tamaño:
2.86 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLÉS.
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista