A putative RNA editing from U to C in a mouse mitochondrial transcript

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dc.contributor.authorVillegas, J.
dc.contributor.authorMüller, I.
dc.contributor.authorArredondo, J.
dc.contributor.authorPinto, R.
dc.contributor.authorBurzio, L.O.
dc.date.accessioned2021-05-27T16:09:40Z
dc.date.available2021-05-27T16:09:40Z
dc.date.issued2002-05
dc.descriptionIndexación Scopuses
dc.description.abstractRecently, we isolated and characterized a new mouse mitochondrial RNA molecule containing the mitochondrial 16S RNA plus 121 nt joined to the 5′ end of the RNA. This fragment arises from the L strand of the same gene and we have named this transcript chimeric RNA. At position 121 of the RNA there is a C, which, according to the sequence of the mitochondrial 16S RNA gene, should be a U. We hypothesized that this RNA is synthesized having a U at position 121, which is later substituted to a C by a putative editing reaction. Based on the presence of sites for the restriction endonucleases RsaI and Fnu4HI around position 121, both forms of the RNA were detected in mouse tissues. To confirm the presence of the non-edited and putative edited RNA, a fragment containing the first 154 nt of the RNA was amplified by RT–PCR and cloned. The substitution of U for C was demonstrated by sequencing these clones. In vitro transcription experiments demonstrated that the substitution of U for C is not due to artifact of amplification or cloning. Moreover, in mitochondria from testis only the non-edited form was found. This, together with other experimental evidence, demonstrated that the base substitution was not due to polymorphism of the mitochondrial 16S RNA gene. This is the first demonstration of a substitution reaction from U to C in a mammalian mitochondrial transcript.es
dc.description.urihttps://academic-oup-com.recursosbiblioteca.unab.cl/nar/article/30/9/1895/1088620
dc.identifier.citationNucleic Acids Research, Volume 30, Issue 9, 1 May 2002, Pages 1895–1901es
dc.identifier.doi10.1093/nar/30.9.1895
dc.identifier.issn0305-1048
dc.identifier.issn1362-4962
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/18962
dc.language.isoenes
dc.publisherOxford University Presses
dc.subjectcytosinees
dc.subjectmitochondrial RNAes
dc.subjectnucleotidees
dc.subjectrestriction endonucleasees
dc.subjectRNA 16Ses
dc.subjecturaciles
dc.titleA putative RNA editing from U to C in a mouse mitochondrial transcriptes
dc.typeArtículoes
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