Th-1 cytotoxic cell-mediated response predominates in the tracheal mucosa following Mycoplasma synoviae infection of MS-H-vaccinated chickens

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dc.contributor.authorOmotainse, Oluwadamilola S.
dc.contributor.authorWawegama, Nadeeka K.
dc.contributor.authorKulappu Arachchige, Sathya N.
dc.contributor.authorCoppo, Mauricio C
dc.contributor.authorVaz, Paola K.
dc.contributor.authorSaliha, Uneeb
dc.contributor.authorBogeski, Mirjana
dc.contributor.authorNoormohammadi, Amir H.
dc.contributor.authorStent, Andrew W.
dc.date.accessioned2025-01-15T20:04:13Z
dc.date.available2025-01-15T20:04:13Z
dc.date.issued2023-12
dc.descriptionIndexación: Scopus
dc.description.abstractMycoplasma synoviae is a pathogen of poultry that causes upper respiratory tract disease. MS-H is a live attenuated temperature-sensitive vaccine that effectively control M. synoviae infection in chickens. However, the mechanisms underpinning protection have not been described previously. In this study, specific-pathogen-free chickens were vaccinated at 3 weeks of age with MS-H vaccine and challenged with field strain M. synoviae 94011/v-18d at 6 weeks of age. Tracheal mucosal inflammation was characterised by the assessment of thickness, histopathological lesions, cellular infiltrates and cytokine transcription. Tracheal lesion scores of unvaccinated-challenged (-V+C) birds were higher than that of vaccinated-challenged (+V+C) birds. +V+C birds displayed early upregulation of IL-4, consistent with a Th-2-skewed response, followed by a later increase in IFN-γ transcription, indicating transition to a Th-1-skewed response. -V+C birds displayed a concurrent early Th-2 and Th-17 response characterised by increase expression of IL-4 and IL-17A respectively, and late T regulatory response characterised by increased IL-10 transcription. +V+C chickens had more cytotoxic T cells (CD8+ T cells) at 7- and 21 days post-challenge (dpc), while -V+C chickens had higher numbers of infiltrating CD4+CD25+ at 7 and 21 dpc. Overall, these observations suggest that the immune response in +V+C chickens had an inflammation characterised by an early Th-2 skewed response followed closely by a Th-1 response and infiltration of cytotoxic T cells, while the response in -V+C chickens was an early Th-2/Th-17-skewed response closely followed by a T regulatory response. © 2023 The Authors
dc.description.urihttps://www-sciencedirect-com.recursosbiblioteca.unab.cl/science/article/pii/S0378113523002754?via%3Dihub
dc.identifier.citationVeterinary Microbiology. Volume 287. December 2023. Article number 109921
dc.identifier.doi10.1016/j.vetmic.2023.109921
dc.identifier.issn0378-1135
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/63031
dc.language.isoen
dc.publisherElsevier B.V.
dc.rights.uriAttribution 4.0 International Deed (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectCellular-response
dc.subjectCytokines
dc.subjectMS-H-vaccine
dc.subjectMycoplasma Synoviae
dc.subjectTracheal-Mucosa
dc.titleTh-1 cytotoxic cell-mediated response predominates in the tracheal mucosa following Mycoplasma synoviae infection of MS-H-vaccinated chickens
dc.typeArtículo
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