Pannexin-1 expression in tumor cells correlates with colon cancer progression and survival

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dc.contributor.authorFierro-Arenas, Aaron
dc.contributor.authorLandskron, Glauben
dc.contributor.authorCamhi-Vainroj, Ilan
dc.contributor.authorBasterrechea, Benjamín
dc.contributor.authorParada-Venegas, Daniela
dc.contributor.authorLobos-González, Lorena
dc.contributor.authorDubois-Camacho, Karen
dc.contributor.authorAraneda, Catalina
dc.contributor.authorRomero, Camila
dc.contributor.authorDomínguez, Antonia
dc.contributor.authorVásquez, Gonzalo
dc.contributor.authorLópez-K, Francisco
dc.contributor.authorAlvarez, Karin
dc.contributor.authorGonzález, Carlos M.*
dc.contributor.authorHager Ribeiro, Carolina
dc.contributor.authorBalboa, Elisa
dc.contributor.authorEugenin, Eliseo
dc.contributor.authorHermoso, Marcela A.
dc.contributor.authorDe la Fuente López, Marjorie
dc.date.accessioned2024-09-11T23:03:40Z
dc.date.available2024-09-11T23:03:40Z
dc.date.issued2024-08
dc.description.abstractAims Pannexin-1 (PANX1) is a hemichannel that releases ATP upon opening, initiating inflammation, cell proliferation, and migration. However, the role of PANX1 channels in colon cancer remains poorly understood, thus constituting the focus of this study. Main methods PANX1 mRNA expression was analyzed using multiple cancer databases. PANX1 protein expression and distribution were evaluated by immunohistochemistry on primary tumor tissue and non-tumor colonic mucosa from colon cancer patients. PANX1 inhibitors (probenecid or 10Panx) were used to assess colon cancer cell lines viability. To study the role of PANX1 in vivo, a subcutaneous xenograft model using HCT116 cells was performed in BALB/c NOD/SCID immunodeficient mice to evaluate tumor growth under PANX1 inhibition using probenecid. Key findings PANX1 mRNA was upregulated in colon cancer tissue compared to non-tumor colonic mucosa. Elevated PANX1 mRNA expression in tumors correlated with worse disease-free survival. PANX1 protein abundance was increased on tumor cells compared to epithelial cells in paired samples, in a cancer stage-dependent manner. In vitro and in vivo experiments indicated that blocking PANX1 reduced cell viability and tumor growth. Significance PANX1 can be used as a biomarker of colon cancer progression and blocking PANX1 channel opening could be used as a potential therapeutic strategy against this disease.
dc.description.accesoabiertoSI
dc.description.agradThis research was funded by The National Research and Development Agency from Chile (Agencia Nacional de Investigación y Desarrollo), Fondecyt de Iniciación #11190990 (MDF), Fondecyt Postdoc #3190931 (GL), 3210367 (KD), Fondecyt de Iniciación #11230904 (GL), #11230689 (EB), Fondecyt Regular #1220702 (MAH), Fondap ACCDiS #15130011 (LLG), Fondecyt #1211223 (LLG), and Internal funding from Universidad Finis Terrae VRICAD-FF2023 (MDF).
dc.description.urihttps://www-sciencedirect-com.recursosbiblioteca.unab.cl/science/article/pii/S0024320524004417
dc.identifier.citationLife Sciences, Volume 351 , 15 August 2024, Article number 122851
dc.identifier.doi10.1016/j.lfs.2024.122851
dc.identifier.folioFondecyt de Iniciación #11190990
dc.identifier.folio Fondecyt Postdoc #3190931
dc.identifier.folioFondecyt Postdoc # 3210367
dc.identifier.folioFondecyt de Iniciación #11230904
dc.identifier.folioFondecyt de Iniciación #11230689
dc.identifier.folioFondecyt Regular #1220702
dc.identifier.folioFondap ACCDiS # 15130011
dc.identifier.folioFondecyt # 1211223
dc.identifier.generoH
dc.identifier.issn1879-0631
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/60124
dc.language.isoes
dc.publisherElsevier Inc.
dc.rights.licenseAttribution-NonCommercial 4.0 International Deed (CC BY-NC 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectPANX1
dc.subjectColon cancer
dc.subjectCancer progression
dc.subjectBiomarker
dc.subjectProbenecid
dc.subjectTherapeutic targeting
dc.titlePannexin-1 expression in tumor cells correlates with colon cancer progression and survival
dc.typeArtículo
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