PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis

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dc.contributor.authorSibu, Simon
dc.contributor.authorSkůpa, Petr
dc.contributor.authorViaene, Tom
dc.contributor.authorZwiewka, Marta
dc.contributor.authorTejos, Ricardo
dc.contributor.authorKlíma, Petr
dc.contributor.authorČarná, Mária
dc.contributor.authorRolčík, Jakub
dc.contributor.authorDe Rycke, Riet
dc.contributor.authorMoreno, Ignacio
dc.contributor.authorDobrev, Petre I.
dc.contributor.authorOrellana, Ariel
dc.contributor.authorZažímalová, Eva
dc.contributor.authorFriml, Jiří
dc.date.accessioned2023-10-25T19:30:33Z
dc.date.available2023-10-25T19:30:33Z
dc.date.issued2016-07
dc.descriptionIndexación: Sopus.es
dc.description.abstractSummary Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate. In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles. The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes. These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport.es
dc.description.urihttps://nph-onlinelibrary-wiley-com.recursosbiblioteca.unab.cl/doi/10.1111/nph.14019
dc.identifier.citationNew Phytologist. Volume 211, Issue 1, Pages 65 - 74. 1 July 2016es
dc.identifier.doi10.1111/nph.14019
dc.identifier.issn0028-646X
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/53621
dc.language.isoenes
dc.publisherBlackwell Publishing Ltdes
dc.subjectAuxines
dc.subjectEndoplasmic Reticulum (ER)es
dc.subjectLateral Rootes
dc.subjectPINes
dc.subjectPlasma Membrane (PM)es
dc.titlePIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsises
dc.typeArtículoes
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